NIFK Antibody (18E148): sc-52904

NIFK Antibody (18E148) is a mouse monoclonal IgG1 antibody that detects NIFK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-NIFK antibody (18E148) is available as the non-conjugated format. NIFK protein plays a crucial role in nitrogen fixation, a process essential for converting atmospheric nitrogen into a form usable by living organisms. Specifically, NIFK encodes the beta subunit of the nitrogenase molybdenum-iron (MoFe) tetramer, which is a key component of the nitrogenase complex involved in this biochemical transformation. NIFK protein is primarily localized in the nucleolus, where NIFK interacts with the fork-head associated domain of the proliferation marker protein Ki-67 in a manner that is both mitosis-specific and dependent on phosphorylation. NIFK presence in various adult tissues suggests additional functions beyond Ki-67 interaction, which is predominantly expressed in proliferating cells. Understanding NIFK role in nitrogen fixation and cellular interactions is vital for exploring potential implications in cellular proliferation and metabolic processes.

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NIFK Antibody (18E148) References:

1. Molecular evolution of nitrogen fixation: the evolutionary history of the nifD, nifK, nifE, and nifN genes.  |  Fani, R., et al. 2000. J Mol Evol. 51: 1-11. PMID: 10903367
2. A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis.  |  Takagi, M., et al. 2001. J Biol Chem. 276: 25386-91. PMID: 11342549
3. The nifk gene is widely expressed in mouse tissues and is up-regulated in denervated hind limb muscle.  |  Magnusson, C., et al. 2003. Cell Biol Int. 27: 469-75. PMID: 12798774
4. Electrophoretic studies on the assembly of the nitrogenase molybdenum-iron protein from the Klebsiella pneumoniae nifD and nifK gene products.  |  White, TC., et al. 1992. J Biol Chem. 267: 24007-16. PMID: 1429737
5. Analysis of Azotobacter vinelandii strains containing defined deletions in the nifD and nifK genes.  |  Li, JG., et al. 1990. J Bacteriol. 172: 5884-91. PMID: 2120192
6. Nucleotide and deduced amino acid sequences of the Klebsiella pneumoniae nifK gene coding for the beta-subunit of nitrogenase MoFe protein.  |  Steinbauer, J., et al. 1988. Nucleic Acids Res. 16: 7199. PMID: 3043382
7. Assessing horizontal transfer of nifHDK genes in eubacteria: nucleotide sequence of nifK from Frankia strain HFPCcI3.  |  Hirsch, AM., et al. 1995. Mol Biol Evol. 12: 16-27. PMID: 7877490
8. Cloning and transcriptional analysis of the nifUHDK genes of Trichodesmium sp. IMS101 reveals stable nifD, nifDK and nifK transcripts.  |  Dominic, B., et al. 1998. Microbiology (Reading). 144 (Pt 12): 3359-3368. PMID: 9884228
9. Nucleotide sequence of nifK and partial sequence of nifD from Frankia species strain FaC1.  |  Ligon, JM. and Nakas, JP. 1988. Nucleic Acids Res. 16: 11843. PMID: 3211766

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIFK Antibody (18E148) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NIFK Antibody (18E148) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIFK Antibody (18E148) Specifications

Alternate Product Name: NIFK

Product Clone Name: 18E148

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 100-200

Epitope Target: NIFK

Epitope Species: human

Product Reactivity/Detection: NIFK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NIFK Antibody (18E148): N/A