nicastrin Antibody (F-3): sc-377214
- nicastrin Antibody (F-3) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of nicastrin of human origin
- recommended for detection of nicastrin of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for nicastrin Antibody (F-3) for WB applications. These reagents are now offered in bundles with nicastrin Antibody (F-3) (see ordering information below).
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nicastrin Antibody (F-3) is a mouse monoclonal IgG1 antibody that detects nicastrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nicastrin plays a crucial role as a type I transmembrane glycoprotein within the presenilin complex, which is essential for the regulated intramembrane proteolysis of various substrates, including the β-Amyloid precursor protein (βAPP) and Notch. The proper functioning of nicastrin is vital for maintaining cellular signaling pathways and preventing the accumulation of neurotoxic β-Amyloid peptides, which are implicated in the pathogenesis of Alzheimer′s disease. Structurally, nicastrin features hydrophilic amino and carboxy-terminal domains, a short hydrophobic transmembrane domain, and potential sites for post-translational modifications such as N-myristoylation and phosphorylation, which may influence stability and interactions with other proteins. Anti-nicastrin antibody (F-3) serves as an invaluable tool for studies related to Alzheimer′s disease and related neurodegenerative conditions by enabling researchers to explore therapeutic strategies aimed at modulating β-Amyloid production.
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nicastrin Antibody (F-3) References:
- Positive and negative regulation of the gamma-secretase activity by nicastrin in a murine model. | Li, J., et al. 2003. J Biol Chem. 278: 33445-9. PMID: 12815056
- Nicastrin gene in familial and sporadic Alzheimer's disease. | Confaloni, A., et al. 2003. Neurosci Lett. 353: 61-5. PMID: 14642438
- Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components. | Zhang, YW., et al. 2005. J Biol Chem. 280: 17020-6. PMID: 15711015
- S-palmitoylation of gamma-secretase subunits nicastrin and APH-1. | Cheng, H., et al. 2009. J Biol Chem. 284: 1373-84. PMID: 19028695
- First nicastrin mutation in PASH (pyoderma gangrenosum, acne and suppurative hidradenitis) syndrome. | Duchatelet, S., et al. 2015. Br J Dermatol. 173: 610-2. PMID: 25601011
- Akt1 phosphorylates Nicastrin to regulate its protein stability and activity. | Jo, EH., et al. 2015. J Neurochem. 134: 799-810. PMID: 25996556
- Loss of Nicastrin from Oligodendrocytes Results in Hypomyelination and Schizophrenia with Compulsive Behavior. | Dries, DR., et al. 2016. J Biol Chem. 291: 11647-56. PMID: 27008863
- Nicastrin haploinsufficiency alters expression of type I interferon-stimulated genes: the relationship to familial hidradenitis suppurativa. | Cao, L., et al. 2019. Clin Exp Dermatol. 44: e118-e125. PMID: 30656721
- Extracellular interface between APP and Nicastrin regulates Aβ length and response to γ-secretase modulators. | Petit, D., et al. 2019. EMBO J. 38: PMID: 31109937
- Expression of nicastrin, NICD1, and Hes1 in NCSTN knockout mice: implications for hidradenitis suppurativa, Alzheimer's, and liver cancer. | Yan, L., et al. 2024. Eur J Med Res. 29: 622. PMID: 39719647
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
nicastrin Antibody (F-3) | sc-377214 | 200 µg/ml | $316.00 | |||
nicastrin Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-540704 | 200 µg Ab; 10 µg BP | $354.00 | |||
nicastrin Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-542312 | 200 µg Ab; 20 µg BP | $354.00 |