NG2 Antibody (F-12): sc-166179
- NG2 Antibody (F-12) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 2023-2322 mapping at the C-terminus of NG2 of human origin
- recommended for detection of NG2 of human origin by WB, IP, IF and ELISA
- See NG2 (LHM 2): sc-53389 for NG2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NG2 Antibody (F-12) for WB applications. These reagents are now offered in bundles with NG2 Antibody (F-12) (see ordering information below).
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SEE ALSO...
NG2 Antibody (F-12) is a mouse monoclonal IgG2a antibody that detects NG2 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NG2, also known as melanoma-associated chondroitin sulfate proteoglycan 4, plays a crucial role in stabilizing cell-substratum interactions, particularly during the early stages of melanoma cell spreading on endothelial basement membranes. This stabilization is vital for facilitating primary melanoma progression, as NG2 enhances the activation of key signaling pathways that are essential for tumor invasion and growth. Notably, the phosphorylation of threonine 2256 in rat NG2 (equivalent to threonine 2252 in human NG2) is significant because NG2 redistribution occurs on the surface of astrocytomas, promoting cell polarization and a marked increase in cell motility. Furthermore, NG2 functions as a co-receptor for spreading and focal contact formation in conjunction with α4 β1 integrin in malignant melanoma cells, underscoring NG2′s importance in cancer biology. In the developing rat embryo, NG2 is expressed on blood vessels, while in the central nervous system, NG2 is found on endothelial cells of microvessels, and outside the CNS, NG2 is present on smooth muscle cells. Additionally, NG2 serves as a novel marker for epidermal stem cells, contributing to organized distribution by promoting clustering, which is essential for maintaining stem cell niches.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NG2 Antibody (F-12) | sc-166179 | 200 µg/ml | $322.00 | |||
NG2 Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-537450 | 200 µg Ab; 10 µg BP | $361.00 | |||
NG2 Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-534722 | 200 µg Ab; 40 µg BP | $361.00 | |||
NG2 Antibody (F-12): m-IgG2a BP-HRP Bundle | sc-548020 | 200 µg Ab; 10 µg BP | $361.00 |