NFRκB CRISPR/Cas9 KO Plasmid (m): sc-433432
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NFRκB CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NFRκB genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NFRκB Antibody (A-12): sc-514977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NFRκB CRISPR/Cas9 KO Plasmid (m) | sc-433432 | 20 µg | $397.00 |
Overview
Mouse Nfrkb encodes NFRκB, a nuclear factor associated with NF-κB–related chromatin and transcriptional regulation that can influence stimulus-responsive gene expression programs. NFRκB has been linked to nuclear protein complexes implicated in DNA-dependent processes, including transcriptional control and maintenance of genome stability. By modulating context-specific expression of inflammatory and stress-response genes, Nfrkb may shape pathways governing cell proliferation, differentiation, and survival. Dysregulation of NF-κB–adjacent regulatory networks is frequently studied in immune dysfunction, inflammatory phenotypes, and cancer-relevant transcriptional states.
NFRκB CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nfrkb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nfrkb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nfrkb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NFRκB protein expression.
This CRISPR knockout system enables efficient generation of Nfrkb-deficient cell models for investigation of NFRκB signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nfrkb exon(s) critical for NFRκB function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nfrkb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NFRκB CRISPR/Cas9 KO Plasmid (m) and NFRκB CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nfrkb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NFRκB HDR Plasmid (m) and NFRκB HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nfrkb homology arms to support homology-directed repair at defined Nfrkb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.