RELA/NFκB p65 Antibody (A-12): sc-514451

NFκB p65 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-286 of the NFκB p65 protein of human origin. Anti-NFκB p65 antibody (A-12) is reactive in species such as mouse, rat, and human, making it suitable for applications in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NFκB p65, also known as RELA, plays a crucial role in regulating gene expression in response to various stimuli such as stress, cytokines, and infections. The p65 subunit forms heterodimers with p50 and, once activated, translocates from the cytoplasm to the nucleus where it binds DNA and influences transcription. Post-translational modifications (PTMs) like phosphorylation and acetylation are essential for modulating NFκB p65 activity. For example, phosphorylation at serine 276 by protein kinase A (PKA) enhances p65′s dimerization with p65 and is critical for nuclear retention and transcriptional activity. Phosphorylation in other regions, such as the transactivation domain (TAD), can affect transcriptional activity and stability. These post-translational modifications are key to regulating immune responses and cellular survival. NFκB p65 (A-12) antibody is designed for detecting p65 in active and modified forms, aiding in the study of signal transduction pathways and transcriptional regulation.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RELA/NFκB p65 Antibody (A-12) has 62 citations in a variety of scientific publications.

Primary antibodies like Anti-RELA/NFκB p65 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RELA/NFκB p65 Antibody (A-12) Specifications

Alternate Product Name: RELA, RelA, p65, NFKB3

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-286

Epitope Target: NFκB p65

Epitope Species: human

Product Reactivity/Detection: NFκB p65

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 62 citations in various scientific publications

Available Conjugates/Forms for RELA/NFκB p65 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred RELA/NFκB p65 antibody is RELA/NFκB p65 Antibody (F-6): sc-8008.