NF45 Antibody (G-3): sc-365068

NF45 Antibody (G-3) is a mouse monoclonal IgG2a antibody that detects NF45 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF45, also known as ILF2, is a crucial transcription factor that plays a significant role in regulating gene expression by forming a heterodimer with NF90 (ILF3). This interaction is particularly important as the NF45/NF90 complex is involved in the regulation of interleukin-2 (IL-2) expression, which is vital for T cell activation and proliferation. The ability of NF45 to bind to the antigen receptor response element (ARRE) within the IL-2 enhancer highlights its essential function in immune responses. Additionally, in neuronal cells, the NF45/NF90 heterodimer has been shown to inhibit the replication of human rhinovirus type 2 by targeting the viral RNA′s 5′ untranslated region, which encodes the internal ribosome entry site (IRES). This dual role in both immune regulation and viral response underscores the importance of NF45 in maintaining cellular homeostasis and responding to external stimuli, making anti-NF45 antibody (G-3) a valuable tool for researchers studying these critical biological processes.

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NF45 Antibody (G-3) References:

1. Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control.  |  Guan, D., et al. 2008. Mol Cell Biol. 28: 4629-41. PMID: 18458058
2. NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response.  |  Graber, TE., et al. 2010. Cell Death Differ. 17: 719-29. PMID: 19893574
3. Nuclear factor NF45 interacts with viral proteins of infectious bursal disease virus and inhibits viral replication.  |  Stricker, RL., et al. 2010. J Virol. 84: 10592-605. PMID: 20702628
4. Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.  |  Todaka, H., et al. 2015. Mol Cell Biol. 35: 2295-308. PMID: 25918244
5. The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators.  |  Nakadai, T., et al. 2015. J Biol Chem. 290: 26832-45. PMID: 26381409
6. Suppression of MicroRNA-7 (miR-7) Biogenesis by Nuclear Factor 90-Nuclear Factor 45 Complex (NF90-NF45) Controls Cell Proliferation in Hepatocellular Carcinoma.  |  Higuchi, T., et al. 2016. J Biol Chem. 291: 21074-21084. PMID: 27519414
7. The properties of the RNA-binding protein NF90 are considerably modulated by complex formation with NF45.  |  Schmidt, T., et al. 2017. Biochem J. 474: 259-280. PMID: 28062840
8. Long non-coding RNA DANCR stabilizes HIF-1α and promotes metastasis by interacting with NF90/NF45 complex in nasopharyngeal carcinoma.  |  Wen, X., et al. 2018. Theranostics. 8: 5676-5689. PMID: 30555573
9. Inducible expression of immediate early genes is regulated through dynamic chromatin association by NF45/ILF2 and NF90/NF110/ILF3.  |  Wu, TH., et al. 2019. PLoS One. 14: e0216042. PMID: 31022259
10. LncRNA LINC00470 promotes proliferation through association with NF45/NF90 complex in hepatocellular carcinoma.  |  Huang, W., et al. 2020. Hum Cell. 33: 131-139. PMID: 31612313

The preferred NF45 antibody is NF45 Antibody (H-4): sc-365283.