NF-L Antibody (NR4): sc-58560

NF-L Antibody (NR4) is a mouse monoclonal IgG1 antibody that detects neurofilament light polypeptide (NF-L) in mouse, rat, and human samples through immunofluorescence (IF) applications. Anti-NF-L antibody (NR4) recognizes a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity of neurons. NF-L (NR4) monoclonal antibody targets proteins primarily located in the axons of neurons, where NF-L contributes to the regulation of axon caliber and overall stability of neuronal architecture. Proper functioning of NF-L is vital for neuronal health, as alterations in expression or phosphorylation status can lead to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease. The dynamic nature of neurofilaments, which includes their ability to interact with other cytoskeletal elements like microtubules and actin filaments, underscores their importance in neuronal function and signaling. NF-L (NR4) monoclonal antibody serves as an essential tool for researchers investigating the role of neurofilaments in health and disease, providing insights into molecular mechanisms underlying neurodegeneration.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NF-L Antibody (NR4) References:

1. Neurofilament proteins NF-L, NF-M and NF-H in brain of patients with Down syndrome and Alzheimer's disease.  |  Bajo, M., et al. 2001. Amino Acids. 21: 293-301. PMID: 11764410
2. Conditional NF-L transgene expression in mice for in vivo analysis of turnover and transport rate of neurofilaments.  |  Millecamps, S., et al. 2007. J Neurosci. 27: 4947-56. PMID: 17475803
3. How the projection domains of NF-L and alpha-internexin determine the conformations of NF-M and NF-H in neurofilaments.  |  Leermakers, FA. and Zhulina, EB. 2010. Eur Biophys J. 39: 1323-34. PMID: 20213320
4. Expression of NF-L and NF-M in fibroblasts reveals coassembly of neurofilament and vimentin subunits.  |  Monteiro, MJ. and Cleveland, DW. 1989. J Cell Biol. 108: 579-93. PMID: 2493000
5. NF-L in cerebrospinal fluid and serum is a biomarker of neuronal damage in an inducible mouse model of neurodegeneration.  |  Brureau, A., et al. 2017. Neurobiol Dis. 104: 73-84. PMID: 28392472
6. The Cytoskeletal Elements MAP2 and NF-L Show Substantial Alterations in Different Stroke Models While Elevated Serum Levels Highlight Especially MAP2 as a Sensitive Biomarker in Stroke Patients.  |  Mages, B., et al. 2021. Mol Neurobiol. 58: 4051-4069. PMID: 33931805
7. Neurofilament Light (NF-L) Chain Protein from a Highly Polymerized Structural Component of the Neuronal Cytoskeleton to a Neurodegenerative Disease Biomarker in the Periphery.  |  Zhao, Y., et al. 2021. HSOA J Alzheimers Neurodegener Dis. 7: PMID: 34881359
8. Neurofilament Light Chain (NF-L) Stimulates Lipid Peroxidation to Neuronal Membrane through Microglia-Derived Ferritin Heavy Chain (FTH) Secretion.  |  Gong, L., et al. 2022. Oxid Med Cell Longev. 2022: 3938940. PMID: 35368870
9. Expression of subtypes NF-L, NF-M and NF-H of neurofilament triplet proteins in the developing rat inner ear.  |  Nishizaki, K. and Anniko, M. 1995. ORL J Otorhinolaryngol Relat Spec. 57: 177-81. PMID: 7478449
10. Changes in neurofilament protein NF-L and NF-H immunoreactivity following kainic acid-induced seizures.  |  Wang, S., et al. 1994. J Neurochem. 62: 739-48. PMID: 8294936

The preferred NF-L antibody is NF-L Antibody (8A1): sc-20012.