NF-L Antibody (F-12): sc-390732

NF-L Antibody (F-12) is a mouse monoclonal IgG1 antibody that detects neurofilament light polypeptide (NF-L) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-L is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity of neurons. NF-L (F-12) antibody targets proteins primarily located in the axons of neurons, where NF-L contributes to the regulation of axon caliber and the overall stability of the neuronal architecture. Proper functioning of NF-L is vital for neuronal health, as alterations in expression or phosphorylation status can lead to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease. Phosphorylation of NF-L at various sites is regulated by multiple kinases, which can influence interactions with other cytoskeletal elements, including microtubules and actin filaments, thereby affecting neuronal signaling and transport processes. Anti-NF-L antibody (F-12) serves as an essential tool for researchers studying the dynamics of neurofilaments and their implications in neurodegenerative conditions, providing insights into the molecular mechanisms underlying these diseases.

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NF-L Antibody (F-12) References:

1. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.  |  Nakamura, Y., et al. 1999. FEBS Lett. 455: 83-6. PMID: 10428477
2. Extra neurofilament NF-L subunits rescue motor neuron disease caused by overexpression of the human NF-H gene in mice.  |  Meier, J., et al. 1999. J Neuropathol Exp Neurol. 58: 1099-110. PMID: 10515233
3. Origin of the beta cells of the islets of Langerhans is further questioned by the expression of neuronal intermediate filament proteins, peripherin and NF-L, in the rat insulinoma RIN5F cell line.  |  Escurat, M., et al. 1991. Dev Neurosci. 13: 424-32. PMID: 1809559
4. Brain injury-associated biomarkers of TGF-beta1, S100B, GFAP, NF-L, tTG, AbetaPP, and tau were concomitantly enhanced and the UPS was impaired during acute brain injury caused by Toxocara canis in mice.  |  Liao, CW., et al. 2008. BMC Infect Dis. 8: 84. PMID: 18573219
5. Identification of serine 473 as a major phosphorylation site in the neurofilament polypeptide NF-L.  |  Xu, ZS., et al. 1990. J Neurosci. 10: 1838-46. PMID: 2162386
6. The complete amino acid sequence of the major mammalian neurofilament protein (NF-L).  |  Geisler, N., et al. 1985. FEBS Lett. 182: 475-8. PMID: 3920075
7. Increased expression of neurofilament subunit NF-L produces morphological alterations that resemble the pathology of human motor neuron disease.  |  Xu, Z., et al. 1993. Cell. 73: 23-33. PMID: 8462100
8. cAMP-dependent phosphorylation of neurofilament proteins NF-L and NF-M inhibits their coassembly into filaments in vitro.  |  Streifel, TD., et al. 1996. Biochem Biophys Res Commun. 222: 646-51. PMID: 8670258
9. Marked decrease of immunolabelled 68 kDa neurofilament (NF-L) proteins in brains of opiate addicts.  |  García-Sevilla, JA., et al. 1997. Neuroreport. 8: 1561-5. PMID: 9189892
10. Domain- and site-specific phosphorylation of bovine NF-L by Rho-associated kinase.  |  Hashimoto, R., et al. 1998. Biochem Biophys Res Commun. 245: 407-11. PMID: 9571164

The preferred NF-L antibody is NF-L Antibody (8A1): sc-20012.