NF-H Antibody (A-12): sc-133165

NF-H Antibody (A-12) is a mouse monoclonal IgG1 antibody that detects neurofilament-H (NF-H) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NF-H is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity of neurons. NF-H is primarily located in the axonal cytoplasm, where NF-H contributes to the formation of neurofilaments, which are essential for the proper functioning of neurons. The presence of neurofilaments is vital for the regulation of axon caliber, which in turn affects the speed of nerve impulse conduction. Additionally, NF-H is subject to extensive post-translational modifications, including phosphorylation, which can influence interactions with other cytoskeletal elements and signaling pathways. Dysregulation of NF-H phosphorylation has been implicated in various neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, highlighting the importance of NF-H in both neuronal health and disease. Anti-NF-H antibody (A-12) is an invaluable tool for researchers studying the dynamics of neurofilament assembly and implications in neurodegenerative conditions.

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NF-H Antibody (A-12) References:

1. Extra neurofilament NF-L subunits rescue motor neuron disease caused by overexpression of the human NF-H gene in mice.  |  Meier, J., et al. 1999. J Neuropathol Exp Neurol. 58: 1099-110. PMID: 10515233
2. Overexpression of neurofilament subunit NF-L and NF-H extends survival of a mouse model for amyotrophic lateral sclerosis.  |  Kong, J. and Xu, Z. 2000. Neurosci Lett. 281: 72-4. PMID: 10686419
3. The C-terminal tail domain of neurofilament protein-H (NF-H) forms the crossbridges and regulates neurofilament bundle formation.  |  Chen, J., et al. 2000. J Cell Sci. 113 Pt 21: 3861-9. PMID: 11034913
4. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury.  |  Anderson, KJ., et al. 2008. J Neurotrauma. 25: 1079-85. PMID: 18729720
5. Phosphorylated neurofilament subunit NF-H as a biomarker for evaluating the severity of spinal cord injury patients, a pilot study.  |  Hayakawa, K., et al. 2012. Spinal Cord. 50: 493-6. PMID: 22270191
6. Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form.  |  Chin, SS. and Liem, RK. 1990. J Neurosci. 10: 3714-26. PMID: 2230956
7. Phosphorylation of the high molecular weight neurofilament protein (NF-H) by Cdk5 and p35.  |  Sun, D., et al. 1996. J Biol Chem. 271: 14245-51. PMID: 8662984
8. Assembly properties of amino- and carboxyl-terminally truncated neurofilament NF-H proteins with NF-L and NF-M in the presence and absence of vimentin.  |  Sun, D., et al. 1997. J Neurochem. 68: 917-26. PMID: 9048736
9. Antagonistic roles of neurofilament subunits NF-H and NF-M against NF-L in shaping dendritic arborization in spinal motor neurons.  |  Kong, J., et al. 1998. J Cell Biol. 140: 1167-76. PMID: 9490729
10. Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M.  |  Veeranna,., et al. 1998. J Neurosci. 18: 4008-21. PMID: 9592082

The preferred NF-H antibody is NF-H Antibody (RNF402): sc-32729.