NF-1 Antibody (D-2): sc-74444

NF-1 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects NF-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NF-1 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NF-1, also known as CTF, plays a crucial role in regulating gene expression by binding to specific DNA sequences, thereby stimulating DNA replication and activating transcription. NF-1 (D-2) monoclonal antibody recognizes a protein that is involved in the transcriptional regulation of numerous genes essential for cellular processes, including cell growth and differentiation. NF-1 has been shown to interact with the adenovirus type 2 promoter and activates transcription of the herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter regions of various eukaryotic genes, such as Ha-Ras, α-globin, HSP 70, GRP 78, and myelin basic protein, highlighting its significance in diverse biological pathways. Two forms of NF-1, resulting from alternative splicing of a single gene, further underscore the complexity and regulatory potential of this transcription factor in cellular function.

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NF-1 Antibody (D-2) References:

1. The role of NF-1 factors in regulation of elastin gene transcription.  |  Degterev, A. and Foster, JA. 1999. Matrix Biol. 18: 295-307. PMID: 10429948
2. Purified cofactors and histone H1 mediate transcriptional regulation by CTF/NF-I.  |  Dusserre, Y. and Mermod, N. 1992. Mol Cell Biol. 12: 5228-37. PMID: 1406693
3. Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a proximal region containing a CCAAT motif which interacts with CTF/NF-I.  |  Wooden, SK., et al. 1991. Mol Cell Biol. 11: 5612-23. PMID: 1656235
4. Isolation of complementary DNAs encoding a cerebellum-enriched nuclear factor I family that activates transcription from the mouse myelin basic protein promoter.  |  Inoue, T., et al. 1990. J Biol Chem. 265: 19065-70. PMID: 1699939
5. The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.  |  Mermod, N., et al. 1989. Cell. 58: 741-53. PMID: 2504497
6. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.  |  Jones, KA., et al. 1987. Cell. 48: 79-89. PMID: 3024847
7. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.  |  Santoro, C., et al. 1988. Nature. 334: 218-24. PMID: 3398920
8. Challenges in the diagnosis of neurofibromatosis type 1 (NF1) in young children facilitated by means of revised diagnostic criteria including genetic testing for pathogenic NF1 gene variants.  |  Kehrer-Sawatzki, H. and Cooper, DN. 2022. Hum Genet. 141: 177-191. PMID: 34928431
9. Two transcriptional activators, CCAAT-box-binding transcription factor and heat shock transcription factor, interact with a human hsp70 gene promoter.  |  Morgan, WD., et al. 1987. Mol Cell Biol. 7: 1129-38. PMID: 3561411
10. Epidemiology and Outcomes of Neurofibromatosis Type 1 (NF-1): Multicenter Tertiary Experience.  |  Almuqbil, M., et al. 2024. J Multidiscip Healthc. 17: 1303-1314. PMID: 38533410
11. Incidence of Ophthalmological Complications in NF-1 Patients Treated with MEK Inhibitors.  |  Hummel, L., et al. 2024. Curr Oncol. 31: 2644-2649. PMID: 38785480
12. Complex organization of CTF/NF-I, C/EBP, and HNF3 binding sites within the promoter of the liver-specific vitellogenin gene.  |  Cardinaux, JR., et al. 1994. J Biol Chem. 269: 32947-56. PMID: 7806524

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NF-1 Antibody (D-2) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-NF-1 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NF-1 Antibody (D-2) Specifications

Alternate Product Name: NFIB

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: NF-1

Epitope Species: human

Product Reactivity/Detection: all NF-1 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for NF-1 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent