Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Neutrophil Elastase CRISPR/Cas9 KO Plasmid (h): sc-400677
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Neutrophil Elastase CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Neutrophil Elastase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Neutrophil Elastase Antibody (G-2): sc-55549
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Neutrophil Elastase CRISPR/Cas9 KO Plasmid (h) | sc-400677 | 20 µg | $397.00 |
Overview
ELANE encodes neutrophil elastase, a granule-associated serine protease released during neutrophil degranulation and NET formation to cleave extracellular matrix proteins and inactivate microbial virulence factors. Its activity is integrated with innate immune signaling, protease–antiprotease balance, and inflammatory cascades that shape leukocyte recruitment and tissue remodeling. Dysregulated neutrophil elastase function contributes to aberrant proteolysis and oxidative/inflammatory stress in airway and vascular microenvironments, and ELANE variants are linked to congenital neutropenia phenotypes with impaired myeloid maturation. As a result, ELANE is widely studied in neutrophil biology, host–pathogen interactions, and mechanisms that couple granulopoiesis to inflammatory tissue injury.
Neutrophil Elastase CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELANE gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELANE together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELANE open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Neutrophil Elastase protein expression.
This CRISPR knockout system enables efficient generation of ELANE-deficient cell models for investigation of Neutrophil Elastase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELANE exon(s) critical for Neutrophil Elastase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELANE genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Neutrophil Elastase CRISPR/Cas9 KO Plasmid (h) and Neutrophil Elastase CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELANE locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Neutrophil Elastase HDR Plasmid (h) and Neutrophil Elastase HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELANE homology arms to support homology-directed repair at defined ELANE target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.