Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
neuroserpin CRISPR/Cas9 KO Plasmid (h): sc-406912
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuroserpin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the neuroserpin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: neuroserpin Antibody (C-9): sc-48360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuroserpin CRISPR/Cas9 KO Plasmid (h) | sc-406912 | 20 µg | $397.00 |
Overview
SERPINI1 encodes neuroserpin, a secreted serine protease inhibitor (serpin) that regulates extracellular proteolysis, most prominently by inhibiting tissue-type plasminogen activator (tPA). By modulating the plasminogen activation cascade, neuroserpin influences pericellular matrix remodeling, synaptic plasticity, and protease-driven signaling in the nervous system. SERPINI1 is highly expressed in neurons and is linked to protein conformational stability and ER quality-control pathways due to its propensity for misfolding under pathogenic variants. Dysregulation of neuroserpin has been associated with neurodegenerative phenotypes and neuronal vulnerability where protease balance and proteostasis are critical.
neuroserpin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINI1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINI1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINI1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish neuroserpin protein expression.
This CRISPR knockout system enables efficient generation of SERPINI1-deficient cell models for investigation of neuroserpin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINI1 exon(s) critical for neuroserpin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINI1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by neuroserpin CRISPR/Cas9 KO Plasmid (h) and neuroserpin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINI1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by neuroserpin HDR Plasmid (h) and neuroserpin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINI1 homology arms to support homology-directed repair at defined SERPINI1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.