Neurogranin Antibody (C-7): sc-515092

Neurogranin Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Neurogranin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Neurogranin antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neurogranin, also known as p17, RC3, and BICKS, is a critical postsynaptic protein predominantly found in the brain, particularly in regions such as the cortex, striatum, hippocampus, thalamus, hypothalamus, and olfactory bulb nuclei. Neurogranin plays a vital role in synaptic plasticity and memory formation by acting as a substrate for protein kinase C (PKC), which phosphorylates Neurogranin, thereby modulating its interaction with calmodulin. This interaction is crucial because Neurogranin binds calmodulin at low calcium levels, influencing calmodulin-dependent nitric oxide synthase activity. Additionally, nitric oxide can post-translationally modify Neurogranin, which reduces calmodulin binding ability and affects PKC phosphorylation. Understanding these interactions and modifications is essential for elucidating the mechanisms underlying synaptic signaling and neuroplasticity, making Neurogranin (C-7) antibody a valuable tool for researchers studying neurological processes and disorders.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Neurogranin Antibody (C-7) References:

1. Localization of the protein kinase C phosphorylation/calmodulin-binding substrate RC3 in dendritic spines of neostriatal neurons.  |  Watson, JB., et al. 1992. Proc Natl Acad Sci U S A. 89: 8581-5. PMID: 1528865
2. Purification and characterization of a brain-specific protein kinase C substrate, neurogranin (p17). Identification of a consensus amino acid sequence between neurogranin and neuromodulin (GAP43) that corresponds to the protein kinase C phosphorylation site and the calmodulin-binding domain.  |  Baudier, J., et al. 1991. J Biol Chem. 266: 229-37. PMID: 1824695
3. Subtractive cDNA cloning of RC3, a rodent cortex-enriched mRNA encoding a novel 78 residue protein.  |  Watson, JB., et al. 1990. J Neurosci Res. 26: 397-408. PMID: 2231781
4. Protein kinase C substrates from bovine brain. Purification and characterization of neuromodulin, a neuron-specific calmodulin-binding protein.  |  Baudier, J., et al. 1989. J Biol Chem. 264: 1824-8. PMID: 2521487
5. The dendritic peptide neurogranin can regulate a calmodulin-dependent target.  |  Martzen, MR. and Slemmon, JR. 1995. J Neurochem. 64: 92-100. PMID: 7528268
6. Characterization of a 7.5-kDa protein kinase C substrate (RC3 protein, neurogranin) from rat brain.  |  Huang, KP., et al. 1993. Arch Biochem Biophys. 305: 570-80. PMID: 8080473
7. Nitric oxide modification of rat brain neurogranin affects its phosphorylation by protein kinase C and affinity for calmodulin.  |  Sheu, FS., et al. 1996. J Biol Chem. 271: 22407-13. PMID: 8798403

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neurogranin Antibody (C-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Neurogranin Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neurogranin Antibody (C-7) Specifications

Alternate Product Name: Neurogranin

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-50

Epitope Target: Neurogranin

Epitope Species: human

Product Reactivity/Detection: Neurogranin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Neurogranin Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin