Neuro D Antibody (A-10): sc-46684

Neuro D Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect human Neuro D protein, also known as NEUROD1 or neuronal differentiation 1. Anti-Neuro D antibody (A-10) targets an epitope spanning amino acids 281-356 of Neuro D protein, ensuring accurate recognition in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Neuro D is a critical basic helix-loop-helix (bHLH) transcription factor involved in neurogenic differentiation, playing an essential role in the development and terminal differentiation of neurons within both the central and peripheral nervous systems. By regulating gene expression, Neuro D is pivotal for cell type determination and maturation, which are fundamental processes for proper neuronal function and overall brain development. Neuro D (A-10) monoclonal antibody is available in various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for diverse experimental requirements. Whether used in non-conjugated form or with specific labels, Neuro D (A-10) antibody effectively facilitates the study of Neuro D′s role in neurogenesis and capacity to induce the differentiation of neuronal precursors into mature neurons, as evidenced by driving neuronal differentiation in model organisms like Xenopus embryos. Reliable and validated, Neuro D (A-10) monoclonal antibody is an indispensable tool for researchers exploring the mechanisms of neuronal development and intricate processes of cell differentiation governed by transcription factors such as Neuro D.

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Neuro D Antibody (A-10) References:

1. ASCL1 and NEUROD1 Reveal Heterogeneity in Pulmonary Neuroendocrine Tumors and Regulate Distinct Genetic Programs.  |  Borromeo, MD., et al. 2016. Cell Rep. 16: 1259-1272. PMID: 27452466
2. Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion.  |  Matsuda, T., et al. 2019. Neuron. 101: 472-485.e7. PMID: 30638745
3. A NeuroD1 AAV-Based Gene Therapy for Functional Brain Repair after Ischemic Injury through In Vivo Astrocyte-to-Neuron Conversion.  |  Chen, YC., et al. 2020. Mol Ther. 28: 217-234. PMID: 31551137
4. SCLC Subtypes Defined by ASCL1, NEUROD1, POU2F3, and YAP1: A Comprehensive Immunohistochemical and Histopathologic Characterization.  |  Baine, MK., et al. 2020. J Thorac Oncol. 15: 1823-1835. PMID: 33011388
5. NeuroD1 induces microglial apoptosis and cannot induce microglia-to-neuron cross-lineage reprogramming.  |  Rao, Y., et al. 2021. Neuron. 109: 4094-4108.e5. PMID: 34875233
6. NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage.  |  Chen, P., et al. 2023. J Neuroinflammation. 20: 261. PMID: 37953259
7. Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein.  |  Lee, JE., et al. 1995. Science. 268: 836-44. PMID: 7754368
8. Interactions of myogenic bHLH transcription factors with calcium-binding calmodulin and S100a (alpha alpha) proteins.  |  Baudier, J., et al. 1995. Biochemistry. 34: 7834-46. PMID: 7794894
9. Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies.  |  Zhang, W., et al. 1995. Genes Dev. 9: 1388-99. PMID: 7797078
10. Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer.  |  Ellenberger, T., et al. 1994. Genes Dev. 8: 970-80. PMID: 7926781
11. Crystal structure of MyoD bHLH domain-DNA complex: perspectives on DNA recognition and implications for transcriptional activation.  |  Ma, PC., et al. 1994. Cell. 77: 451-9. PMID: 8181063
12. Recognition by Max of its cognate DNA through a dimeric b/HLH/Z domain.  |  Ferré-D'Amaré, AR., et al. 1993. Nature. 363: 38-45. PMID: 8479534

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neuro D Antibody (A-10) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-Neuro D Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neuro D Antibody (A-10) Specifications

Alternate Product Name: Neuro D (neuronal differentiation) 1

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 281-356

Epitope Target: Neuro D

Epitope Species: human

Product Reactivity/Detection: Neuro D

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for Neuro D Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent