Neurabin-II Antibody (D-7): sc-373974

Neurabin-II Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Neurabin-II protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Neurabin-II (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neurabin-II, also known as spinophilin, plays a crucial role in the central nervous system by interacting with actin and protein phosphatase 1 (PP-1) within dendritic spines. The gene encoding human Neurabin-II is located on chromosome 17q21-q22, and structural features include an F-actin binding domain at the N-terminus, a coiled-coil structure at the C-terminus, a PDZ domain in the middle region, and a domain that interacts with transmembrane proteins. These structural characteristics enable Neurabin-II to bundle actin filaments in vitro and localize to cortical sites of actin filaments and areas of active membrane remodeling in vivo. Furthermore, Neurabin-II forms a complex with the catalytic subunit of PP-1, modulating enzymatic activity, which is essential for regulating the activity of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors. By doing so, Neurabin-II influences both glutamatergic synaptic transmission and dendritic morphology, highlighting Neurabin-II′s importance in synaptic plasticity and neuronal communication. Additionally, the synergistic interactions between spinophilin and the human tumor suppressor ARF suggest a potential role for Neurabin-II in cell growth regulation, making Neurabin-II a valuable target for research in neurobiology and cancer studies.

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Neurabin-II Antibody (D-7) References:

1. Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction.  |  Sakisaka, T., et al. 1999. Oncogene. 18: 1609-17. PMID: 10102631
2. In vivo dynamics of the F-actin-binding protein neurabin-II.  |  Stephens, DJ. and Banting, G. 2000. Biochem J. 345 Pt 2: 185-94. PMID: 10620493
3. Spinophilin regulates the formation and function of dendritic spines.  |  Feng, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 9287-92. PMID: 10922077
4. The human tumor suppressor arf interacts with spinophilin/neurabin II, a type 1 protein-phosphatase-binding protein.  |  Vivo, M., et al. 2001. J Biol Chem. 276: 14161-9. PMID: 11278317
5. The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits.  |  Terry-Lorenzo, RT., et al. 2002. J Biol Chem. 277: 27716-24. PMID: 12016225
6. Identification of neurabin II as a novel doublecortin interacting protein.  |  Tsukada, M., et al. 2003. Mech Dev. 120: 1033-43. PMID: 14550532
7. The identification of a second actin-binding region in spinophilin/neurabin II.  |  Barnes, AP., et al. 2004. Brain Res Mol Brain Res. 124: 105-13. PMID: 15135218
8. Doublecortin association with actin filaments is regulated by neurabin II.  |  Tsukada, M., et al. 2005. J Biol Chem. 280: 11361-8. PMID: 15632197
9. Neurabin II mediates doublecortin-dephosphorylation on actin filaments.  |  Tsukada, M., et al. 2006. Biochem Biophys Res Commun. 343: 839-47. PMID: 16564023
10. Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines.  |  Allen, PB., et al. 1997. Proc Natl Acad Sci U S A. 94: 9956-61. PMID: 9275233
11. Neurabin-II/spinophilin. An actin filament-binding protein with one pdz domain localized at cadherin-based cell-cell adhesion sites.  |  Satoh, A., et al. 1998. J Biol Chem. 273: 3470-5. PMID: 9452470
12. Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.  |  Ide, N., et al. 1998. Biochem Biophys Res Commun. 244: 258-62. PMID: 9514910

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neurabin-II Antibody (D-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Neurabin-II Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neurabin-II Antibody (D-7) Specifications

Alternate Product Name: Neurabin-II

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 261-430

Epitope Target: Neurabin-II

Epitope Species: human

Product Reactivity/Detection: Neurabin-II

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Neurabin-II Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin