Neurabin-II Antibody (D-7) recommended for detection of Neurabin-II of human origin by WB, IP, IF, IHC(P) and ELISA

Neurabin-II Antibody (D-7): sc-373974

Neurabin-II Antibody (D-7) is rated 4.7 out of 5 by 6.
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Datasheets

    • Anti-Neurabin-II Antibody (D-7) is a mouse monoclonal IgG1 κ Neurabin-II antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 261-430 mapping within an internal region of Neurabin-II of human origin
    • Neurabin-II Antibody (D-7) is recommended for detection of Neurabin-II of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Neurabin-II Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Neurabin-II (D-7): sc-373974.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Neurabin-II Antibody (D-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with Neurabin-II Antibody (D-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Why does neurabin II run at ~130kD, when the sequence predicts an 89kDa protein? Seems like a large difference.

Asked by: mmoritz
Thank you for your question. The observed molecular weight is significantly higher than the predicted molecular weight. Please refer to the following publication as a reference for an approximately 140 kDa observed molecular weight for Neurabin-II, also known as Spinophilin, PMID: 9275233.
Answered by: Technical Support
Date published: 2019-11-22

I am using Neurabin-II (D-7): sc-373974 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Great IF staining cortical neurons Stains cultured cortical neurons beautifully. Looks just like actin stain in the growth cone. First SPN antibody I have had that works. These are 36hr cultures of mouse cortical neurons. (Yes, it works on mouse) Use methanol fixation- (I do mine after PFA fixation) and the antibody at 1:100. Without methanol you can see a bit but its super faint.
Date published: 2017-12-08
Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-02-07
Rated 4 out of 5 by from Good for Western Blot in human brain We have tested this antibody in human brain homogenates by Western Blot and we can visualise a clear band at 120 kDa approximately.
Date published: 2016-12-07
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclear Superb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-02-15
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