Neu1 Antibody (F-8): sc-166824

Neu1 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Neu1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Neu1 Antibody (F-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neu1 protein encodes the lysosomal enzyme neuraminidase, which plays a crucial role in the catabolism of sialic acid-containing glycoproteins and glycolipids, thereby influencing cellular signaling and interactions. This enzymatic activity is vital for maintaining cellular homeostasis and proper functioning of the immune system, as sialic acid residues are involved in modulating cell recognition and adhesion processes. Neu1 is localized primarily in the lysosome, where Neu1 forms a heterotrimeric complex with beta-galactosidase and cathepsin A, also known as the protective protein, which is essential for the stability and activity of these enzymes. Deficiencies in Neu1 can lead to severe neurodegenerative lysosomal storage disorders, such as sialidosis and galactosialidosis, characterized by a range of symptoms including neurological disturbances and skeletal malformations. The human Neu1 gene is located on chromosome 6p21.33 and encodes a lysosomal protein that is found on the inner side of the plasma membrane and in intracellular vesicles, highlighting Neu1′s importance in cellular metabolism and disease pathology.

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Neu1 Antibody (F-8) References:

1. Splice donor site mutation in the lysosomal neuraminidase gene causing exon skipping and complete loss of enzyme activity in a sialidosis patient.  |  Penzel, R., et al. 2001. FEBS Lett. 501: 135-8. PMID: 11470272
2. Prenatal diagnosis and fetal pathology in a Turkish family harboring a novel nonsense mutation in the lysosomal alpha-N-acetyl-neuraminidase (sialidase) gene.  |  Sergi, C., et al. 2001. Hum Genet. 109: 421-8. PMID: 11702224
3. Systemic and neurologic abnormalities distinguish the lysosomal disorders sialidosis and galactosialidosis in mice.  |  de Geest, N., et al. 2002. Hum Mol Genet. 11: 1455-64. PMID: 12023988
4. Identification of a CTL4/Neu1 fusion transcript in a sialidosis patient.  |  Uhl, J., et al. 2002. FEBS Lett. 521: 19-23. PMID: 12067718
5. Therapeutic Potential of Neu1 in Alzheimer's Disease Via the Immune System.  |  Khan, A., et al. 2021. Am J Alzheimers Dis Other Demen. 36: 1533317521996147. PMID: 33719595
6. NEU1 Regulates Mitochondrial Energy Metabolism and Oxidative Stress Post-myocardial Infarction in Mice via the SIRT1/PGC-1 Alpha Axis.  |  Guo, Z., et al. 2022. Front Cardiovasc Med. 9: 821317. PMID: 35548408
7. NEU1-A Unique Therapeutic Target for Alzheimer's Disease.  |  Khan, A. and Sergi, CM. 2022. Front Pharmacol. 13: 902259. PMID: 35847014
8. Targeting intracellular Neu1 for coronavirus infection treatment.  |  Yang, D., et al. 2023. iScience. 26: 106037. PMID: 36714013
9. Structure of the immunoregulatory sialidase NEU1.  |  Gorelik, A., et al. 2023. Sci Adv. 9: eadf8169. PMID: 37205763
10. Lysosomal sialidase NEU1, its intracellular properties, deficiency, and use as a therapeutic agent.  |  Itoh, K. and Tsukimoto, J. 2023. Glycoconj J. 40: 611-619. PMID: 38147151
11. The role of sialidase Neu1 in respiratory diseases.  |  Mei, S., et al. 2024. Respir Res. 25: 134. PMID: 38500102
12. Neuraminidase-1 (NEU1): Biological Roles and Therapeutic Relevance in Human Disease.  |  Du, J., et al. 2024. Curr Issues Mol Biol. 46: 8031-8052. PMID: 39194692

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Neu1 Antibody (F-8) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Neu1 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Neu1 Antibody (F-8) Specifications

Alternate Product Name: Neuraminidase 1

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 116-415

Epitope Target: Neu1

Epitope Species: human

Product Reactivity/Detection: Neu1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Neu1 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin