ErbB2/HER2 Antibody (F-11): sc-7301

Neu Antibody (F-11) is a mouse monoclonal IgG2a kappa light chain antibody raised against human Neu (ErbB-2/HER2), a critical member of the epidermal growth factor receptor family involved in cell growth regulation. Neu mouse monoclonal antibody (F-11) recognizes an epitope within the Neu immunogen sequence and demonstrates broad species cross-reactivity, detecting Neu in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-Neu monoclonal antibody (F-11) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. Neu functions as a sophisticated signal transducer through precise subcellular localization at the cell membrane, where Neu operates as a receptor tyrosine kinase to rapidly respond to extracellular signals. This strategic positioning enables Neu to form either homodimers with other Neu molecules or heterodimers with additional EGFR family members upon ligand binding, initiating downstream signaling cascades that regulate cell proliferation and differentiation. When Neu expression becomes dysregulated, particularly through overexpression, the resulting enhanced signaling can drive aggressive forms of breast cancer development. This association between Neu overexpression and cancer progression makes Neu both a valuable prognostic biomarker and a promising therapeutic target. Through these diverse cellular roles, Neu (F-11) antibody serves as an essential tool for investigating cancer biology mechanisms and developing targeted therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ErbB2/HER2 Antibody (F-11) has 70 citations in a variety of scientific publications.

Primary antibodies like Anti-ErbB2/HER2 Antibody (F-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ErbB2/HER2 Antibody (F-11) Specifications

Alternate Product Name: HER2

Product Clone Name: F-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1238-1255

Epitope Target: Neu

Epitope Species: human

Product Reactivity/Detection: Neu gp185

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 70 citations in various scientific publications

Available Conjugates/Forms for ErbB2/HER2 Antibody (F-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred antibody is Antibody (): sc-8.