Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Net CRISPR/Cas9 KO Plasmid (h): sc-405945
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Net CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Net genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Net Antibody (11G9): sc-134401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Net CRISPR/Cas9 KO Plasmid (h) | sc-405945 | 20 µg | $397.00 |
Overview
ELK3 (Net) is an ETS family transcription factor that integrates MAPK/ERK signaling to regulate context-dependent gene expression programs controlling angiogenesis, cell migration, and differentiation. Net functions as a transcriptional repressor or activator depending on phosphorylation state and cofactor recruitment, linking growth factor cues to immediate-early and vascular gene networks. Through modulation of pathways governing endothelial behavior and epithelial–mesenchymal plasticity, ELK3 is frequently studied in models of tumor progression, hypoxia-associated responses, and vascular remodeling. Dysregulated ELK3 activity has been associated with altered invasive phenotypes and aberrant angiogenic signaling in multiple cancer contexts, supporting its use as a mechanistic node in oncogenic transcriptional circuitry.
Net CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELK3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELK3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELK3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Net protein expression.
This CRISPR knockout system enables efficient generation of ELK3-deficient cell models for investigation of Net signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELK3 exon(s) critical for Net function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELK3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Net CRISPR/Cas9 KO Plasmid (h) and Net CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELK3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Net HDR Plasmid (h) and Net HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELK3 homology arms to support homology-directed repair at defined ELK3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.