Net Antibody (11G9): sc-134401

Net Antibody (11G9) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Net protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Net antibody (11G9) is available as the non-conjugated form. Net protein, also known as ERP or SAP-2, plays a crucial role in Ras signaling pathways, which are essential for various cellular processes, including growth, differentiation, and survival. Located primarily in the nucleus, Net contains two nuclear localization signals and a nuclear export signal, allowing Net to shuttle between the nucleus and cytoplasm in response to cellular stress. This shuttling is vital for Net′s function as a transcriptional regulator; under stress conditions, Net is exported from the nucleus, where Net acts as a transcriptional repressor. However, when activated by Ras/ERK signaling, Net can transition into a transcriptional activator, highlighting Net′s dual role in gene regulation. Net′s ability to interact with other signaling proteins, such as ERK and p38, further underscores Net′s importance in modulating transcriptional responses to extracellular signals. Anti-Net antibody (11G9) enables researchers to gain insights into the complex regulatory mechanisms of gene expression influenced by Ras signaling and the dynamic nature of transcriptional control.

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Net Antibody (11G9) References:

1. The net repressor is regulated by nuclear export in response to anisomycin, UV, and heat shock.  |  Ducret, C., et al. 1999. Mol Cell Biol. 19: 7076-87. PMID: 10490644
2. The ternary complex factor Net contains two distinct elements that mediate different responses to MAP kinase signalling cascades.  |  Ducret, C., et al. 2000. Oncogene. 19: 5063-72. PMID: 11042694
3. Proteins of the ETS family with transcriptional repressor activity.  |  Mavrothalassitis, G. and Ghysdael, J. 2000. Oncogene. 19: 6524-32. PMID: 11175368
4. The transcription factor Net regulates the angiogenic switch.  |  Zheng, H., et al. 2003. Genes Dev. 17: 2283-97. PMID: 12975317
5. Ets ternary complex transcription factors.  |  Buchwalter, G., et al. 2004. Gene. 324: 1-14. PMID: 14693367
6. The tumor suppressor p53 inhibits Net, an effector of Ras/extracellular signal-regulated kinase signaling.  |  Nakade, K., et al. 2004. Mol Cell Biol. 24: 1132-42. PMID: 14729959
7. Loss of net as repressor leads to constitutive increased c-fos transcription in cervical cancer cells.  |  van Riggelen, J., et al. 2005. J Biol Chem. 280: 3286-94. PMID: 15548518
8. Comparative analysis of the ternary complex factors Elk-1, SAP-1a and SAP-2 (ERP/NET).  |  Price, MA., et al. 1995. EMBO J. 14: 2589-601. PMID: 7540136
9. Net, a new ets transcription factor that is activated by Ras.  |  Giovane, A., et al. 1994. Genes Dev. 8: 1502-13. PMID: 7958835