nestin Antibody (7A3): sc-101541

Nestin Antibody (7A3) is a rat monoclonal IgG2b antibody that detects nestin in mouse samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Nestin is a crucial intermediate filament protein predominantly expressed in embryonic central nervous system progenitor cells, playing a vital role in structural integrity and function. Nestin′s dynamic presence in the intermediate filament network is particularly important during muscle development, where nestin interacts with other filament proteins such as Desmin and Vimentin to form heterodimer coiled-coil structures that contribute to the assembly of 10 nm filaments. Nestin′s localization is critical; deletion in the intermediate filament consensus rod domain can disrupt localization in central nervous system precursor cells and radial glial cells, which may have implications for neurodevelopmental processes. Furthermore, nestin serves as a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells, especially during periods of rapid growth, highlighting nestin′s significance in cancer biology. During neuron differentiation, nestin is found at growth cones, suggesting a role in guiding axon elongation. Additionally, nestin expression is observed in specific regions of the rat adrenal gland, such as the zona fasciculata and zona reticularis, as well as in dermatomal cells and myoblasts during early stages of myogenesis, underscoring nestin′s diverse functional roles across different tissues.

The preferred nestin antibody is nestin Antibody (10c2): sc-23927.