nestin Antibody (5C93): sc-71665

Nestin Antibody (5C93) is a mouse monoclonal IgG1 antibody that detects nestin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Nestin is a crucial intermediate filament protein predominantly found in embryonic central nervous system progenitor cells, where nestin plays a vital role in maintaining structural integrity and function during development. Nestin′s dynamic presence is also significant in muscle development, as nestin polymerizes with other intermediate filament proteins like Desmin and Vimentin, forming a robust network essential for cellular architecture. Nestin (5C93) antibody recognizes protein structures that co-assemble with Vimentin or α-internexin into heterodimer coiled-coil structures, contributing to the formation of 10 nm intermediate filaments critical for cellular resilience. Deletion of the intermediate filament consensus rod domain in nestin can disrupt localization in central nervous system precursor cells and radial glial cells, highlighting nestin′s importance in cellular positioning and function. Nestin serves as a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during periods of rapid growth, making nestin a valuable target for research in neurodevelopment and cancer biology. During neuronal differentiation, nestin localizes to growth cones, suggesting a role in guiding axon elongation, essential for proper neural network formation. In rat adrenal gland, nestin expression is observed in the zona fasciculata and zona reticularis, as well as in dermatomal cells and myoblasts during early stages of myogenesis, further underscoring nestin′s diverse roles in various tissues. Anti-nestin antibody (5C93) is an indispensable tool for researchers investigating the complexities of cellular development and differentiation.

The preferred nestin antibody is nestin Antibody (10c2): sc-23927.