nestin Antibody (5C93): sc-71665

Nestin Antibody (5C93) is a mouse monoclonal IgG1 antibody that detects nestin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Nestin is a crucial intermediate filament protein predominantly found in embryonic central nervous system progenitor cells, where nestin plays a vital role in maintaining structural integrity and function during development. Nestin′s dynamic presence is also significant in muscle development, as nestin polymerizes with other intermediate filament proteins like Desmin and Vimentin, forming a robust network essential for cellular architecture. Nestin (5C93) antibody recognizes protein structures that co-assemble with Vimentin or α-internexin into heterodimer coiled-coil structures, contributing to the formation of 10 nm intermediate filaments critical for cellular resilience. Deletion of the intermediate filament consensus rod domain in nestin can disrupt localization in central nervous system precursor cells and radial glial cells, highlighting nestin′s importance in cellular positioning and function. Nestin serves as a marker for neuroepithelial stem cells, glioma cells, and tumor endothelial cells during periods of rapid growth, making nestin a valuable target for research in neurodevelopment and cancer biology. During neuronal differentiation, nestin localizes to growth cones, suggesting a role in guiding axon elongation, essential for proper neural network formation. In rat adrenal gland, nestin expression is observed in the zona fasciculata and zona reticularis, as well as in dermatomal cells and myoblasts during early stages of myogenesis, further underscoring nestin′s diverse roles in various tissues. Anti-nestin antibody (5C93) is an indispensable tool for researchers investigating the complexities of cellular development and differentiation.

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nestin Antibody (5C93) References:

1. Development of mice with brain-specific deletion of floxed glud1 (glutamate dehydrogenase 1) using cre recombinase driven by the nestin promoter.  |  Karaca, M. and Maechler, P. 2014. Neurochem Res. 39: 456-9. PMID: 23595828
2. Nestin is a marker of lung remodeling secondary to myocardial infarction and type I diabetes in the rat.  |  Chabot, A., et al. 2015. J Cell Physiol. 230: 170-9. PMID: 24915827
3. Nestin regulates proliferation and invasion of gastrointestinal stromal tumor cells by altering mitochondrial dynamics.  |  Wang, J., et al. 2016. Oncogene. 35: 3139-50. PMID: 26434586
4. Nestin-positive/SOX2-negative cells mediate adult neurogenesis of nigral dopaminergic neurons in mice.  |  Albright, JE., et al. 2016. Neurosci Lett. 615: 50-4. PMID: 26806039
5. Nestin-Based Reporter Transgenic Mouse Lines.  |  Mignone, J., et al. 2016. Methods Mol Biol. 1453: 7-14. PMID: 27431241
6. Nestin suppression attenuates invasive potential of endometrial cancer cells by downregulating TGF-β signaling pathway.  |  Bokhari, AA., et al. 2016. Oncotarget. 7: 69733-69748. PMID: 27626172
7. Nestin expression is differently regulated between odontoblasts and the subodontoblastic layer in mice.  |  Nakatomi, M., et al. 2018. Histochem Cell Biol. 149: 383-391. PMID: 29445893
8. Nestin in the epididymis is expressed in vascular wall cells and is regulated during postnatal development and in case of testosterone deficiency.  |  Reckmann, AN., et al. 2018. PLoS One. 13: e0194585. PMID: 29874225
9. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension.  |  Bhagwani, AR., et al. 2019. PLoS One. 14: e0213890. PMID: 30883593

The preferred nestin antibody is nestin Antibody (10c2): sc-23927.