NERF CRISPR/Cas9 KO Plasmid (h): sc-405891
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NERF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NERF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NERF Antibody (224C4a): sc-130632
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NERF CRISPR/Cas9 KO Plasmid (h) | sc-405891 | 20 µg | $397.00 |
Overview
ELF2 encodes NERF, an ETS family transcription factor that binds purine-rich DNA motifs to regulate gene expression programs controlling hematopoietic differentiation, immune cell activation, and lineage-specific transcriptional networks. NERF participates in RNA polymerase II–dependent transcription and cooperates with other transcription factors and chromatin regulators to modulate promoter and enhancer activity. Dysregulated ELF2/NERF signaling has been linked to altered proliferation and survival pathways in hematologic contexts, supporting its study in leukemogenesis and immune dysregulation. As a nuclear regulatory factor, NERF is also used as a node for dissecting ETS-driven transcriptional circuitry and downstream pathway remodeling.
NERF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NERF protein expression.
This CRISPR knockout system enables efficient generation of ELF2-deficient cell models for investigation of NERF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELF2 exon(s) critical for NERF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NERF CRISPR/Cas9 KO Plasmid (h) and NERF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NERF HDR Plasmid (h) and NERF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELF2 homology arms to support homology-directed repair at defined ELF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.