Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NEPH3 CRISPR/Cas9 KO Plasmid (h): sc-406190
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEPH3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NEPH3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NEPH3 Antibody (G-12): sc-515104
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEPH3 CRISPR/Cas9 KO Plasmid (h) | sc-406190 | 20 µg | $397.00 |
Overview
KIRREL2 encodes NEPH3, an immunoglobulin superfamily adhesion molecule enriched at specialized cell–cell junctions where it supports formation and maintenance of slit diaphragm–like structures and epithelial barrier organization. NEPH3 participates in contact-dependent signaling and cytoskeletal remodeling through interactions with scaffold proteins that coordinate actin dynamics and junctional complex stability. In kidney-relevant contexts, NEPH3-associated complexes are linked to podocyte architecture, filtration barrier integrity, and cellular responses to mechanical or inflammatory stress. Dysregulation of NEPH family adhesion networks has been associated with glomerular pathology and altered epithelial differentiation, making KIRREL2 a useful target for studying junctional signaling and tissue homeostasis.
NEPH3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the KIRREL2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the KIRREL2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the KIRREL2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NEPH3 protein expression.
This CRISPR knockout system enables efficient generation of KIRREL2-deficient cell models for investigation of NEPH3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting KIRREL2 exon(s) critical for NEPH3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple KIRREL2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NEPH3 CRISPR/Cas9 KO Plasmid (h) and NEPH3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the KIRREL2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NEPH3 HDR Plasmid (h) and NEPH3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by KIRREL2 homology arms to support homology-directed repair at defined KIRREL2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.