NEPH3 Antibody (G-12): sc-515104
- NEPH3 Antibody (G-12) is a mouse monoclonal IgG1 κ NEPH3 antibody provided at 200 µg/ml
- raised against amino acids 546-600 mapping near the C-terminus of NEPH3 of human origin
- NEPH3 Antibody (G-12) is recommended for detection of NEPH3 of human origin by WB, IP, IF and ELISA
- Anti-NEPH3 Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NEPH3 Antibody (G-12) for WB applications. These reagents are now offered in bundles with NEPH3 Antibody (G-12) (see ordering information below).
QUICK LINKS
NEPH3 Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects NEPH3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NEPH3 Antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NEPH3, also known as nephrin-like protein 3, NLG1, FILTRIN, or KIRREL2 (kin of IRRE like 2), is a 708 amino acid protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. NEPH3 is characterized by five extracellular immunoglobulin-like repeats, a transmembrane domain, and a cytoplasmic domain containing a stretch of nine conserved residues. NEPH3 is primarily localized to the interpodocyte filtration slit in the kidney and the Langerhans islet beta cells in the pancreas, playing a crucial role in maintaining the glomerular filtration barrier in the kidney and contributing to pancreatic development. NEPH3 undergoes various post-translational modifications, including glycosylation, which are essential for proper function and stability. NEPH3 downregulation has been implicated in several proteinuric diseases, highlighting significance in renal health. Five isoforms of NEPH3 exist due to alternative splicing events, which may contribute to diverse functional roles in different tissues.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NEPH3 Antibody (G-12) References:
- Filtrin is a novel member of nephrin-like proteins. | Ihalmo, P., et al. 2003. Biochem Biophys Res Commun. 300: 364-70. PMID: 12504092
- Kirrel2, a novel immunoglobulin superfamily gene expressed primarily in beta cells of the pancreatic islets. | Sun, C., et al. 2003. Genomics. 82: 130-42. PMID: 12837264
- Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord. | Minaki, Y., et al. 2005. Neurosci Res. 52: 250-62. PMID: 15927725
- Podocyte-associated proteins FAT, alpha-actinin-4 and filtrin are expressed in Langerhans islets of the pancreas. | Rinta-Valkama, J., et al. 2007. Mol Cell Biochem. 294: 117-25. PMID: 16841182
- A neuronal identity code for the odorant receptor-specific and activity-dependent axon sorting. | Serizawa, S., et al. 2006. Cell. 127: 1057-69. PMID: 17129788
- Expression of filtrin in human glomerular diseases. | Ihalmo, P., et al. 2007. Nephrol Dial Transplant. 22: 1903-9. PMID: 17442742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEPH3 Antibody (G-12) | sc-515104 | 200 µg/ml | $316.00 | |||
NEPH3 Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-531287 | 200 µg Ab; 10 µg BP | $354.00 | |||
NEPH3 Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-524935 | 200 µg Ab, 40 µg BP | $354.00 | |||
NEPH3 Antibody (G-12): m-IgG1 BP-HRP Bundle | sc-544469 | 200 µg Ab; 20 µg BP | $354.00 | |||
NEPH3 Antibody (G-12) AC | sc-515104 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NEPH3 Antibody (G-12) HRP | sc-515104 HRP | 200 µg/ml | $316.00 | |||
NEPH3 Antibody (G-12) FITC | sc-515104 FITC | 200 µg/ml | $330.00 | |||
NEPH3 Antibody (G-12) PE | sc-515104 PE | 200 µg/ml | $343.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 488 | sc-515104 AF488 | 200 µg/ml | $357.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 546 | sc-515104 AF546 | 200 µg/ml | $357.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 594 | sc-515104 AF594 | 200 µg/ml | $357.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 647 | sc-515104 AF647 | 200 µg/ml | $357.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 680 | sc-515104 AF680 | 200 µg/ml | $357.00 | |||
NEPH3 Antibody (G-12) Alexa Fluor® 790 | sc-515104 AF790 | 200 µg/ml | $357.00 |