Nek7 Antibody (B-5) recommended for detection of Nek7 of mouse, rat and human origin by WB, IP, IF and ELISA

Nek7 Antibody (B-5): sc-393539

Nek7 Antibody (B-5) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-Nek7 Antibody (B-5) is a mouse monoclonal IgG1 (kappa light chain) Nek7 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 1-26 at the N-terminus of Nek7 of human origin
    • Anti-Nek7 Antibody (B-5) is recommended for detection of Nek7 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-Nek7 Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-393539 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nek7 (B-5): sc-393539.

What application is the blocking peptide sc-393539 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! This antibody is recommended for detection of Nek7 of mouse, rat, human, equine, canine, bovine and porcine origin by WB, IP, IF and ELISA.
Date published: 2017-02-08
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmic Produced superb immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-11
Rated 5 out of 5 by from Satisfactory Western Blot data of Nek7 expression Satisfactory Western Blot data of Nek7 expression in non-transfected 293T, mouse Nek7 transfected 293T, HeLa, Jurkat and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-10-18
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