Nek5 Antibody (G-12): sc-515457

Nek5 Antibody (G-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nek5 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nek5 (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nek5 protein, also known as NimA-related protein kinase 5, plays a crucial role in cell cycle regulation, particularly in mitosis transition. Nek5 belongs to the serine/threonine protein kinase superfamily and consists of 708 amino acids, featuring a protein kinase domain that facilitates ATP-dependent phosphorylation of target proteins. This phosphorylation is vital for various cellular processes, including cell division and apoptosis, as Nek5 modulates the activity of numerous substrates involved in these pathways. Proper functioning of Nek5 is essential for maintaining cellular homeostasis and ensuring accurate cell cycle progression, making Nek5 a significant target for research in cancer biology and cell cycle regulation.

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Nek5 Antibody (G-12) References:

1. Mitotic kinases: the key to duplication, segregation, and cytokinesis errors, chromosomal instability, and oncogenesis.  |  Li, JJ. and Li, SA. 2006. Pharmacol Ther. 111: 974-84. PMID: 16603252
2. Mitotic regulation by NIMA-related kinases.  |  O'regan, L., et al. 2007. Cell Div. 2: 25. PMID: 17727698
3. Members of the plant NIMA-related kinases are involved in organ development and vascularization in poplar, Arabidopsis and rice.  |  Vigneault, F., et al. 2007. Plant J. 51: 575-88. PMID: 17886359
4. Cdk1, Plks, Auroras, and Neks: the mitotic bodyguards.  |  Salaun, P., et al. 2008. Adv Exp Med Biol. 617: 41-56. PMID: 18497029
5. The NIMA-family kinase, Nek1 affects the stability of centrosomes and ciliogenesis.  |  White, MC. and Quarmby, LM. 2008. BMC Cell Biol. 9: 29. PMID: 18533026
6. Knockdown of lncRNA XIST inhibits hypoxia-induced glycolysis, migration and invasion through regulating miR-381-3p/NEK5 axis in nasopharyngeal carcinoma.  |  Zhao, CH., et al. 2020. Eur Rev Med Pharmacol Sci. 24: 2505-2517. PMID: 32196601
7. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.  |  Hanks, SK., et al. 1988. Science. 241: 42-52. PMID: 3291115
8. Polyglutamylase activity of tubulin tyrosine ligase-like 4 is negatively regulated by the never in mitosis gene A family kinase never in mitosis gene A -related kinase 5.  |  Melo-Hanchuk, TD. and Kobarg, J. 2021. World J Biol Chem. 12: 38-51. PMID: 34084286
9. The NEK family of serine/threonine kinases as a biomarker for cancer.  |  Panchal, NK. and Evan Prince, S. 2023. Clin Exp Med. 23: 17-30. PMID: 35037094
10. Evidence for a NIMA-like mitotic pathway in vertebrate cells.  |  Lu, KP. and Hunter, T. 1995. Cell. 81: 413-24. PMID: 7736593
11. Mitotic destruction of the cell cycle regulated NIMA protein kinase of Aspergillus nidulans is required for mitotic exit.  |  Pu, RT. and Osmani, SA. 1995. EMBO J. 14: 995-1003. PMID: 7889945
12. The NIMA kinase: a mitotic regulator in Aspergillus nidulans and vertebrate cells.  |  Lu, KP. and Hunter, T. 1995. Prog Cell Cycle Res. 1: 187-205. PMID: 9552363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nek5 Antibody (G-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Nek5 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nek5 Antibody (G-12) Specifications

Alternate Product Name: Nek5

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 413-491

Epitope Target: Nek5

Epitope Species: human

Product Reactivity/Detection: Nek5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Nek5 Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin