Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NEIL1 CRISPR/Cas9 KO Plasmid (h): sc-403778
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NEIL1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NEIL1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NEIL1 Antibody (D-1): sc-271164
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NEIL1 CRISPR/Cas9 KO Plasmid (h) | sc-403778 | 20 µg | $397.00 |
Overview
NEIL1 (endonuclease VIII-like 1) is a DNA glycosylase/AP lyase that initiates base excision repair by recognizing and excising oxidized DNA lesions, including damaged pyrimidines, and processing abasic sites to promote strand incision. It functions within oxidative stress response and genome maintenance pathways, coordinating with downstream BER enzymes to restore DNA integrity during replication and transcription. NEIL1 activity influences mutation accumulation and chromosomal stability, linking its dysregulation to mechanisms underlying carcinogenesis and other disorders associated with oxidative DNA damage. As a nuclear repair factor, NEIL1 is frequently studied in the context of replication-associated repair, mitochondrial/nuclear genome stability, and cellular responses to reactive oxygen species.
NEIL1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NEIL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NEIL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NEIL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NEIL1 protein expression.
This CRISPR knockout system enables efficient generation of NEIL1-deficient cell models for investigation of NEIL1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NEIL1 exon(s) critical for NEIL1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NEIL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NEIL1 CRISPR/Cas9 KO Plasmid (h) and NEIL1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NEIL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NEIL1 HDR Plasmid (h) and NEIL1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NEIL1 homology arms to support homology-directed repair at defined NEIL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.