NEIL1 Antibody (B-1) recommended for detection of NEIL1 of human origin by WB, IP, IF and ELISA

NEIL1 Antibody (B-1): sc-271167

NEIL1 Antibody (B-1) is rated 5.0 out of 5 by 1.
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Datasheets

    • NEIL1 Antibody (B-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-49 mapping at the N-terminus of NEIL1 of human origin
    • recommended for detection of NEIL1 of human origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-271167 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

Can NEIL1 Antibody (D-1)X (sc-271164X) be used for "Shift-Western blotting" (combination of gel shift assay and Western blot transfer, PNAS, 1993, v.90, 2574)?

Asked by: vlads5
Thank you for your question. This antibody has not yet been tested for shift-Western blot so that is not a recommended application at this time. It is recommended and warrantied for Western blot, but we have no information about Shift-Western.
Answered by: Tech Service
Date published: 2020-02-24

What is the recommended starting dilution for Western blot for sc-271167: NEIL1 (B-1) monoclonal antibody?

Asked by: DefinitelyNotMatt
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Nice Western blot data of NEIL1 expression in non Nice Western blot data of NEIL1 expression in non-transfected and human NEIL1 transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-07-30
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