NEDD1 Antibody (39-J) recommended for detection of NEDD1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

NEDD1 Antibody (39-J): sc-100961

NEDD1 Antibody (39-J) is rated 4.2 out of 5 by 5.
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Datasheets

    • NEDD1 Antibody (39-J) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 100 µg/ml
    • raised against recombinant NEDD1 of human origin
    • recommended for detection of NEDD1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Can sc-100961: NEDD1 (39-J) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-100961: NEDD1 (39-J) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great for IF staining. This monoclonal worked great for IF labeling of centrosomes in human HEK293 and in CHO cells at a dilution of 1:500.
Date published: 2017-07-14
Rated 4 out of 5 by from Nedd1 antibody works well in cultured cells Nedd1 antibody works well in cultured cells use at 1:100 in Methanol fix. Works okay in tissue but not so well in western blot.
Date published: 2016-07-27
Rated 3 out of 5 by from Positive results in WB with mouse NEDD1 transfected Positive results in WB with mouse NEDD1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Strong Strong, clean bands in western blot using U-2 OS whole cell lysate and HeLa nuclear extract. -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Great immunoperoxidase membrane staining Great immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-02-14
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