NDUFS3 Antibody (D-4): sc-374282

NDUFS3 Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDUFS3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NDUFS3 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NDUFS3, or NADH dehydrogenase ubiquinone iron-sulfur protein 3, plays a critical role as one of approximately 45 subunits that constitute complex I of the oxidative phosphorylation electron transport chain, which is essential for ATP production in mitochondria. NDUFS3 is located within the inner mitochondrial membrane, where NDUFS3 facilitates the transfer of electrons from NADH to ubiquinone, a key step in cellular respiration. Proper functioning of NDUFS3 is vital for maintaining energy homeostasis, and NDUFS3 dysfunction can lead to severe metabolic disorders, including optic atrophy, Leigh syndrome, and complex I deficiency. Notably, NDUFS3 contains a highly conserved phosphorylation site for casein kinase II, which may influence NDUFS3 activity and interactions within the complex. Anti-NDUFS3 antibody (D-4) effectively detects NDUFS3 across multiple applications, making anti-NDUFS3 antibody (D-4) an invaluable tool for researchers studying mitochondrial function and related pathologies.

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NDUFS3 Antibody (D-4) References:

1. Mutant NDUFS3 subunit of mitochondrial complex I causes Leigh syndrome.  |  Bénit, P., et al. 2004. J Med Genet. 41: 14-7. PMID: 14729820
2. Leigh syndrome associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFS1 gene.  |  Martín, MA., et al. 2005. Arch Neurol. 62: 659-61. PMID: 15824269
3. A high-fat diet coordinately downregulates genes required for mitochondrial oxidative phosphorylation in skeletal muscle.  |  Sparks, LM., et al. 2005. Diabetes. 54: 1926-33. PMID: 15983191
4. Ultrasound evaluation of peritoneal catheter tunnel in catheter related infections in CAPD.  |  Karahan, OI., et al. 2005. Int Urol Nephrol. 37: 363-6. PMID: 16142572
5. Cell biological consequences of mitochondrial NADH: ubiquinone oxidoreductase deficiency.  |  Smeitink, JA., et al. 2004. Curr Neurovasc Res. 1: 29-40. PMID: 16181064
6. A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: ubiquinone oxidoreductase (complex I).  |  Flemming, D., et al. 2005. J Mol Microbiol Biotechnol. 10: 208-22. PMID: 16645316
7. Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I).  |  Gostimskaya, IS., et al. 2006. Biochim Biophys Acta. 1757: 1155-61. PMID: 16777054
8. Determination of the cDNA sequence for the human mitochondrial 75-kDa Fe-S protein of NADH-coenzyme Q reductase.  |  Chow, W., et al. 1991. Eur J Biochem. 201: 547-50. PMID: 1935949
9. cDNA sequence and chromosomal localization of the remaining three human nuclear encoded iron sulphur protein (IP) subunits of complex I: the human IP fraction is completed.  |  Loeffen, J., et al. 1998. Biochem Biophys Res Commun. 247: 751-8. PMID: 9647766

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NDUFS3 Antibody (D-4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-NDUFS3 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NDUFS3 Antibody (D-4) Specifications

Alternate Product Name: NDUFS3

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-248

Epitope Target: NDUFS3

Epitope Species: human

Product Reactivity/Detection: NDUFS3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for NDUFS3 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin