NDST1 CRISPR/Cas9 KO Plasmid (h): sc-404078

Overview

NDST1 (N-deacetylase/N-sulfotransferase 1) is a key Golgi-resident enzyme that initiates and propagates N-sulfation during heparan sulfate proteoglycan biosynthesis, shaping the sulfation patterns that determine ligand binding specificity. By controlling heparan sulfate structure, NDST1 modulates extracellular matrix organization and cell-surface co-receptor function that influence growth factor and morphogen signaling pathways, including FGF, WNT, Hedgehog, and VEGF axes. These sulfation-dependent interactions affect cell adhesion, migration, and differentiation programs that are central to developmental and tissue homeostasis. Dysregulated NDST1 activity and altered heparan sulfate composition have been associated with diverse pathophysiology, including tumor microenvironment remodeling, fibrosis-related signaling, and neurodevelopmental phenotypes, making NDST1 a useful target for mechanistic studies of glycocalyx-driven signaling.

NDST1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NDST1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NDST1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NDST1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NDST1 protein expression.

This CRISPR knockout system enables efficient generation of NDST1-deficient cell models for investigation of NDST1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting NDST1 exon(s) critical for NDST1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple NDST1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by NDST1 CRISPR/Cas9 KO Plasmid (h) and NDST1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NDST1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by NDST1 HDR Plasmid (h) and NDST1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NDST1 homology arms to support homology-directed repair at defined NDST1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.