Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NDRG3 CRISPR/Cas9 KO Plasmid (m): sc-424349
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NDRG3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NDRG3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NDRG3 Antibody (H-11): sc-514561
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDRG3 CRISPR/Cas9 KO Plasmid (m) | sc-424349 | 20 µg | $397.00 |
Overview
Ndrg3 encodes NDRG3, a cytoplasmic protein in the NDRG family implicated in regulation of cell growth, differentiation, and stress adaptation. In mouse systems, NDRG3 has been linked to metabolic signaling and hypoxia-associated pathways, including lactate-dependent stabilization mechanisms that can influence downstream transcriptional programs. Altered NDRG3 activity has been studied in contexts of proliferative control and cellular survival, making it relevant for investigating how metabolic cues shape tissue homeostasis. These functions position NDRG3 as a useful node for dissecting pathway cross-talk between nutrient sensing, hypoxic responses, and cell fate decisions.
NDRG3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ndrg3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ndrg3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ndrg3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NDRG3 protein expression.
This CRISPR knockout system enables efficient generation of Ndrg3-deficient cell models for investigation of NDRG3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ndrg3 exon(s) critical for NDRG3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ndrg3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NDRG3 CRISPR/Cas9 KO Plasmid (m) and NDRG3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ndrg3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NDRG3 HDR Plasmid (m) and NDRG3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ndrg3 homology arms to support homology-directed repair at defined Ndrg3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.