NDEL1 Antibody (D-5): sc-365094
- NDEL1 Antibody (D-5) is a mouse monoclonal IgG1 κ NDEL1 antibody provided at 200 µg/ml
- raised against amino acids 181-245 mapping within an internal region of NDEL1 of human origin
- NDEL1 Antibody (D-5) is recommended for detection of NDEL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NDEL1 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NDEL1 Antibody (D-5) for WB applications. These reagents are now offered in bundles with NDEL1 Antibody (D-5) (see ordering information below).
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NDEL1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NDEL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NDEL1 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The NudE-like protein (Ndel1) plays a crucial role in cellular processes, particularly in the organization of microtubules and the positioning of the nucleus during cell division. Ndel1 is predominantly expressed in the testis, brain, heart, hypothalamus, liver, lung, spleen, and stomach, specifically localized at the interphase centrosome and mitotic spindle. Ndel1 positively regulates minus-end directed dynein, and Ndel1 interaction with LIS1 is essential for sustaining dynein function, which in turn impacts microtubule organization and neuronal positioning. Ndel1 undergoes phosphorylation during mitosis, facilitating the tethering of dynactin and dynein to the mother centriole, thereby anchoring microtubules. Disruption of Ndel1 function in the developing neocortex can lead to impaired neuronal positioning and a disconnection between the centrosome and nucleus, which may contribute to mitochondrial transport dysfunction and is associated with psychiatric conditions such as lissencephaly and schizophrenia.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NDEL1 Antibody (D-5) References:
- NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. | Niethammer, M., et al. 2000. Neuron. 28: 697-711. PMID: 11163260
- Human Nudel and NudE as regulators of cytoplasmic dynein in poleward protein transport along the mitotic spindle. | Yan, X., et al. 2003. Mol Cell Biol. 23: 1239-50. PMID: 12556484
- Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning. | Shu, T., et al. 2004. Neuron. 44: 263-77. PMID: 15473966
- Inhibition of NUDEL (nuclear distribution element-like)-oligopeptidase activity by disrupted-in-schizophrenia 1. | Hayashi, MA., et al. 2005. Proc Natl Acad Sci U S A. 102: 3828-33. PMID: 15728732
- Subcellular targeting of DISC1 is dependent on a domain independent from the Nudel binding site. | Brandon, NJ., et al. 2005. Mol Cell Neurosci. 28: 613-24. PMID: 15797709
- NudEL targets dynein to microtubule ends through LIS1. | Li, J., et al. 2005. Nat Cell Biol. 7: 686-90. PMID: 15965467
- Complete loss of Ndel1 results in neuronal migration defects and early embryonic lethality. | Sasaki, S., et al. 2005. Mol Cell Biol. 25: 7812-27. PMID: 16107726
- Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration. | Toyo-Oka, K., et al. 2005. Hum Mol Genet. 14: 3113-28. PMID: 16203747
- Nudel contributes to microtubule anchoring at the mother centriole and is involved in both dynein-dependent and -independent centrosomal protein assembly. | Guo, J., et al. 2006. Mol Biol Cell. 17: 680-9. PMID: 16291865
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NDEL1 Antibody (D-5) | sc-365094 | 200 µg/ml | $322.00 | |||
NDEL1 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-529309 | 200 µg Ab; 10 µg BP | $361.00 | |||
NDEL1 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-522136 | 200 µg Ab, 40 µg BP | $361.00 | |||
NDEL1 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-543445 | 200 µg Ab; 20 µg BP | $361.00 | |||
NDEL1 Antibody (D-5) AC | sc-365094 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NDEL1 Antibody (D-5) HRP | sc-365094 HRP | 200 µg/ml | $322.00 | |||
NDEL1 Antibody (D-5) FITC | sc-365094 FITC | 200 µg/ml | $336.00 | |||
NDEL1 Antibody (D-5) PE | sc-365094 PE | 200 µg/ml | $349.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 488 | sc-365094 AF488 | 200 µg/ml | $364.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 546 | sc-365094 AF546 | 200 µg/ml | $364.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 594 | sc-365094 AF594 | 200 µg/ml | $364.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 647 | sc-365094 AF647 | 200 µg/ml | $364.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 680 | sc-365094 AF680 | 200 µg/ml | $364.00 | |||
NDEL1 Antibody (D-5) Alexa Fluor® 790 | sc-365094 AF790 | 200 µg/ml | $364.00 |