NCoA-3 Antibody (F-2): sc-5305

NCoA-3 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects NCoA-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NCoA-3 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. NCoA-3, also known as receptor-associated coactivator 3, plays a crucial role in the regulation of gene expression as a transcriptional coactivator for steroid hormone receptors, thyroid hormone receptors, and retinoic acid receptors. NCoA-3 interaction with nuclear receptors is vital for transcriptional activation of target genes, particularly in hormone-responsive elements. NCoA-3 is implicated in breast and ovarian cancers, where elevated expression is associated with estrogen receptor positivity, highlighting importance in cancer biology and potential as a therapeutic target. NCoA-3 interaction with other coactivators, such as GRIP1 and SRC-1, further underscores a role in modulating transcriptional responses to hormonal signals, making NCoA-3 significant in both normal physiology and disease states.

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NCoA-3 Antibody (F-2) References:

1. The transcriptional co-activator p/CIP (NCoA-3) is up-regulated by STAT6 and serves as a positive regulator of transcriptional activation by STAT6.  |  Arimura, A., et al. 2004. J Biol Chem. 279: 31105-12. PMID: 15145939
2. Nuclear Receptor Coactivator NCOA3 Regulates UV Radiation-Induced DNA Damage and Melanoma Susceptibility.  |  de Semir, D., et al. 2021. Cancer Res. 81: 2956-2969. PMID: 33766890
3. NCOA3 is a critical oncogene in thyroid cancer via the modulation of major signaling pathways.  |  Li, Y., et al. 2022. Endocrine. 75: 149-158. PMID: 34251576
4. NCOA3, a new player in melanoma susceptibility and a therapeutic target.  |  Bezrookove, V. and Kashani-Sabet, M. 2022. Cancer Gene Ther. 29: 399-401. PMID: 35322161
5. AIB1/SRC-3/NCOA3 function in estrogen receptor alpha positive breast cancer.  |  Kiliti, AJ., et al. 2023. Front Endocrinol (Lausanne). 14: 1250218. PMID: 37711895
6. GTF2H4 regulates partial EndMT via NF-κB activation through NCOA3 phosphorylation in ischemic diseases.  |  Fang, Z., et al. 2024. Innovation (Camb). 5: 100565. PMID: 38379791
7. Sequence and characterization of a coactivator for the steroid hormone receptor superfamily.  |  Oñate, SA., et al. 1995. Science. 270: 1354-7. PMID: 7481822
8. The nuclear hormone receptor gene superfamily.  |  Ribeiro, RC., et al. 1995. Annu Rev Med. 46: 443-53. PMID: 7598477
9. GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors.  |  Hong, H., et al. 1996. Proc Natl Acad Sci U S A. 93: 4948-52. PMID: 8643509
10. The transcriptional co-activator p/CIP binds CBP and mediates nuclear-receptor function.  |  Torchia, J., et al. 1997. Nature. 387: 677-84. PMID: 9192892
11. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2.  |  Li, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 8479-84. PMID: 9238002
12. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer.  |  Anzick, SL., et al. 1997. Science. 277: 965-8. PMID: 9252329

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NCoA-3 Antibody (F-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-NCoA-3 Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NCoA-3 Antibody (F-2) Specifications

Alternate Product Name: NCOA3

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 455-851

Epitope Target: NCoA-3

Epitope Species: mouse

Product Reactivity/Detection: NCoA-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for NCoA-3 Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent