NCK1/2 Antibody (G-3): sc-365802
- NCK1/2 Antibody (G-3) is a mouse monoclonal IgG1 κ NCK1/2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 359-377 near the C-terminus of NCK1 of human origin
- NCK1/2 Antibody (G-3) is recommended for detection of NCK1 and NCK2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NCK1/2 Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NCK1/2 Antibody (G-3) for WB applications. These reagents are now offered in bundles with NCK1/2 Antibody (G-3) (see ordering information below).
NCK1/2 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NCK1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NCK1/2 (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The NCK family of SH2/SH3 adaptor proteins, comprising NCK1 (NCKα) and NCK2 (NCKβ), plays a crucial role in linking tyrosine kinase signaling pathways, such as those activated by epidermal growth factor (EGF) and platelet-derived growth factor (PDGF), to downstream signaling proteins. NCK1 interacts with various signaling molecules, including p21-activated kinase (PAK), which is a key regulator of the actin cytoskeleton. This interaction is vital for cellular processes such as migration and morphology, as NCK1 mediates the connection between PAK and RAC, a small GTPase involved in cytoskeletal dynamics. Additionally, NCK2 binds to human PDGFR-β at tyrosine 1009, and NCK2 overexpression can inhibit PDGF-induced membrane ruffling and lamellipod formation, highlighting its regulatory role in cell motility. Human NCK1 and NCK2 genes are located on chromosomes 3q21 and 2q12, respectively, and their expression is modulated by various growth factor receptors, cell surface antigens, and adhesion molecules, underscoring their significance in cellular signaling and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NCK1/2 Antibody (G-3) References:
- The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains. | Frese, S., et al. 2006. J Biol Chem. 281: 18236-45. PMID: 16636066
- The adaptor protein Nck2 mediates Slit1-induced changes in cortical neuron morphology. | Round, JE. and Sun, H. 2011. Mol Cell Neurosci. 47: 265-73. PMID: 21600986
- Nck2 promotes human melanoma cell proliferation, migration and invasion in vitro and primary melanoma-derived tumor growth in vivo. | Labelle-Côté, M., et al. 2011. BMC Cancer. 11: 443. PMID: 21992144
- A Study of Interleukin-2 Production and Nck Adaptor Molecule Gene Expression in Systemic Lupus Erythematosus Patients. | Towiwat, P. and Buttham, B. 2017. J Med Assoc Thai. 100: 396-402. PMID: 29911835
- Proteomic Analysis of NCK1/2 Adaptors Uncovers Paralog-specific Interactions That Reveal a New Role for NCK2 in Cell Abscission During Cytokinesis. | Jacquet, K., et al. 2018. Mol Cell Proteomics. 17: 1979-1990. PMID: 30002203
- NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. | Dubrac, A., et al. 2018. Nat Commun. 9: 3463. PMID: 30150707
- Aristolochic acid inhibits Slit2-induced migration and tube formation via inactivation of Robo1/Robo2-NCK1/NCK2 signaling pathway in human umbilical vein endothelial cells. | Guan, T., et al. 2019. Toxicol Lett. 300: 51-58. PMID: 30381256
- Complementary Nck1/2 Signaling in Podocytes Controls α Actinin-4-Mediated Actin Organization, Adhesion, and Basement Membrane Composition. | Martin, CE., et al. 2022. J Am Soc Nephrol. 33: 1546-1567. PMID: 35906089
- Endothelial NCK2 promotes atherosclerosis progression in male but not female Nck1-null atheroprone mice. | Bywaters, BC., et al. 2022. Front Cardiovasc Med. 9: 955027. PMID: 36035930
- Nck1 regulates the in vitro development of human regulatory T cells through AKT pathway. | Nuiyen, A., et al. 2025. Clin Exp Immunol.. PMID: 39963999
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NCK1/2 Antibody (G-3) | sc-365802 | 200 µg/ml | $322.00 | |||
NCK1/2 Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-529473 | 200 µg Ab; 10 µg BP | $361.00 | |||
NCK1/2 Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-522374 | 200 µg Ab, 40 µg BP | $361.00 | |||
NCK1/2 Antibody (G-3) AC | sc-365802 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NCK1/2 Antibody (G-3) HRP | sc-365802 HRP | 200 µg/ml | $322.00 | |||
NCK1/2 Antibody (G-3) FITC | sc-365802 FITC | 200 µg/ml | $336.00 | |||
NCK1/2 Antibody (G-3) PE | sc-365802 PE | 200 µg/ml | $349.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 488 | sc-365802 AF488 | 200 µg/ml | $364.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 546 | sc-365802 AF546 | 200 µg/ml | $364.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 594 | sc-365802 AF594 | 200 µg/ml | $364.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 647 | sc-365802 AF647 | 200 µg/ml | $364.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 680 | sc-365802 AF680 | 200 µg/ml | $364.00 | |||
NCK1/2 Antibody (G-3) Alexa Fluor® 790 | sc-365802 AF790 | 200 µg/ml | $364.00 | |||
NCK1/2 (G-3) Neutralizing Peptide | sc-365802 P | 100 µg/0.5 ml | $69.00 |