NC2α Antibody (H-3): sc-374336

NC2α Antibody (H-3) is a mouse monoclonal IgM kappa antibody that detects NC2 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-NC2 alpha antibody (H-3) is available as the non-conjugated form. NC2 alpha protein, part of the negative cofactor 2 complex, plays a crucial role in regulating RNA polymerase II transcription by binding to the TATA-binding protein (TBP) and inhibiting essential transcription factors such as TFIIA and TFIIB. This regulation is vital for maintaining proper gene expression, as NC2 alpha ensures transcription is activated only when necessary, preventing inappropriate gene activation that could lead to cellular dysfunction. The NC2 complex, composed of NC2α and NC2β subunits, interacts with TBP-promoter complexes through histone fold domains, influencing DNA conformation and modulating transcriptional activity. Direct DNA binding capacity and correlation with transcriptional activity highlight NC2 alpha′s importance in gene regulation, making NC2α (H-3) monoclonal antibody an essential tool for researchers studying transcriptional mechanisms and their implications in various biological processes.

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NC2α Antibody (H-3) References:

1. Human TATA-binding protein-related factor-2 (hTRF2) stably associates with hTFIIA in HeLa cells.  |  Teichmann, M., et al. 1999. Proc Natl Acad Sci U S A. 96: 13720-5. PMID: 10570139
2. A single point mutation in TFIIA suppresses NC2 requirement in vivo.  |  Xie, J., et al. 2000. EMBO J. 19: 672-82. PMID: 10675336
3. Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.  |  Geisberg, JV., et al. 2001. Mol Cell Biol. 21: 2736-42. PMID: 11283253
4. A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.  |  Goppelt, A., et al. 1996. EMBO J. 15: 3105-16. PMID: 8670811
5. Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae.  |  Goppelt, A. and Meisterernst, M. 1996. Nucleic Acids Res. 24: 4450-5. PMID: 8948634

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NC2α Antibody (H-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NC2α Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NC2α Antibody (H-3) Specifications

Alternate Product Name: NC2α

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 47-82

Epitope Target: NC2α

Epitope Species: human

Product Reactivity/Detection: NC2α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NC2α Antibody (H-3): P: Blocking Peptide, X: TransCruz Reagent