NAT-1/2 Antibody (G-5): sc-137204
- NAT-1/2 Antibody (G-5) is a mouse monoclonal IgG1 κ NAT-1/2 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 261-290 at the C-terminus of NAT-1 of human origin
- NAT-1/2 Antibody (G-5) is recommended for detection of NAT-1 of human origin and NAT-2 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-NAT-1/2 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NAT-1/2 Antibody (G-5) for WB and IHC(P) applications. These reagents are now offered in bundles with NAT-1/2 Antibody (G-5) (see ordering information below).
NAT-1/2 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NAT-1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NAT-1/2 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Arylamine N-acetyltransferases (NAT-1 and NAT-2) are crucial enzymes that catalyze the N- or O-acetylation of heterocyclic and arylamine substrates, playing a significant role in the detoxification of a wide range of drugs. NAT-1 and NAT-2 involvement in drug metabolism has potential implications for cancer risk, as certain alleles associated with high N-acetyltransferase activity have been linked to increased risk of colon and urinary bladder cancers through bioactivation of several known carcinogens. NAT-1 and NAT-2 are located in the cytoplasm, where these enzymes actively participate in detoxifying arylamine and hydrazine drugs. N-acetylation polymorphism is determined by NAT activity levels in liver tissues, correlating with efficacy and toxicity of amine-containing drugs. Human NAT-1 serves as the functional homolog of rodent NAT-2, while human NAT-2 corresponds to rodent NAT-1, highlighting the evolutionary conservation and functional significance of these enzymes across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NAT-1/2 Antibody (G-5) References:
- Polymorphisms of glutathione-S-transferase and arylamine N-acetyltransferase enzymes and susceptibility to colorectal cancer. | Kiss, I., et al. 2004. Anticancer Res. 24: 3965-70. PMID: 15736440
- Constitutional short telomeres are strong genetic susceptibility markers for bladder cancer. | Broberg, K., et al. 2005. Carcinogenesis. 26: 1263-71. PMID: 15746160
- Are polymorphisms of N-acetyltransferase genes susceptible to primary liver cancer in Luoyang, China? | Zhang, XF., et al. 2005. World J Gastroenterol. 11: 1457-62. PMID: 15770721
- Lack of association between endometriosis and N-acetyl transferase 1 (NAT1) and 2 (NAT2) polymorphisms in a Japanese population. | Deguchi, M., et al. 2005. J Soc Gynecol Investig. 12: 208-13. PMID: 15784508
- N-acetyltransferase is involved in baicalein-induced N-acetylation of 2-aminofluorene and DNA-2-aminofluorene adduct formation in human leukemia HL-60 cells. | Li, YC., et al. 2005. In Vivo. 19: 399-405. PMID: 15796204
- [Morbidity and mortality in the pediatric service of Banqui (Central African Republic) during the year 1990. Implications for public health]. | Lanckriet, C., et al. 1992. Ann Pediatr (Paris). 39: 125-30. PMID: 1580526
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NAT-1/2 Antibody (G-5) | sc-137204 | 200 µg/ml | $322.00 | |||
NAT-1/2 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-528874 | 200 µg Ab; 10 µg BP | $361.00 | |||
NAT-1/2 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-521450 | 200 µg Ab, 40 µg BP | $361.00 | |||
NAT-1/2 Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-543184 | 200 µg Ab; 20 µg BP | $361.00 | |||
NAT-1/2 Antibody (G-5) AC | sc-137204 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NAT-1/2 Antibody (G-5) HRP | sc-137204 HRP | 200 µg/ml | $322.00 | |||
NAT-1/2 Antibody (G-5) FITC | sc-137204 FITC | 200 µg/ml | $336.00 | |||
NAT-1/2 Antibody (G-5) PE | sc-137204 PE | 200 µg/ml | $349.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 488 | sc-137204 AF488 | 200 µg/ml | $364.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 546 | sc-137204 AF546 | 200 µg/ml | $364.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 594 | sc-137204 AF594 | 200 µg/ml | $364.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 647 | sc-137204 AF647 | 200 µg/ml | $364.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 680 | sc-137204 AF680 | 200 µg/ml | $364.00 | |||
NAT-1/2 Antibody (G-5) Alexa Fluor® 790 | sc-137204 AF790 | 200 µg/ml | $364.00 | |||
NAT-1/2 (G-5) Neutralizing Peptide | sc-137204 P | 100 µg/0.5 ml | $69.00 |