Na+ CP type VIIIα CRISPR/Cas9 KO Plasmid (m): sc-422823
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Na+ CP type VIIIα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Na+ CP type VIIIα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Na+ CP type VIIIα Antibody (W-78): sc-81884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Na+ CP type VIIIα CRISPR/Cas9 KO Plasmid (m) | sc-422823 | 20 µg | $397.00 |
Overview
Scn8a encodes the mouse voltage-gated sodium channel Na⁺ CP type VIIIα, a key determinant of action potential initiation and high-frequency firing in neurons. By mediating rapid Na⁺ influx at the axon initial segment and nodes of Ranvier, it supports neuronal excitability and signal propagation and functionally couples to membrane repolarization and ion homeostasis pathways. Altered SCN8A activity is widely used to model mechanisms of channelopathy-related hyperexcitability, including seizure susceptibility, sensory processing abnormalities, and neurodevelopmental phenotypes. This target is therefore relevant for studying how sodium channel gating and distribution shape circuit-level physiology and downstream transcriptional programs.
Na+ CP type VIIIα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scn8a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scn8a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scn8a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Na+ CP type VIIIα protein expression.
This CRISPR knockout system enables efficient generation of Scn8a-deficient cell models for investigation of Na+ CP type VIIIα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scn8a exon(s) critical for Na+ CP type VIIIα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scn8a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Na+ CP type VIIIα CRISPR/Cas9 KO Plasmid (m) and Na+ CP type VIIIα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scn8a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Na+ CP type VIIIα HDR Plasmid (m) and Na+ CP type VIIIα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scn8a homology arms to support homology-directed repair at defined Scn8a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.