NARG1 Antibody (D-7): sc-365931
- NARG1 Antibody (D-7) is a mouse monoclonal IgG1 κ NARG1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 325-370 mapping within an internal region of NARG1 of human origin
- NARG1 Antibody (D-7) is recommended for detection of NARG1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-NARG1 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NARG1 Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with NARG1 Antibody (D-7) (see ordering information below).
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NARG1 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects NARG1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NARG1 Antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NARG1 protein, also known as NMDA (N-methyl-d-aspartate) receptor-regulated gene 1, NATH (N-terminal acetyltransferase), TBDN100 (tubedown-1), or Ga19 (Gastric cancer antigen Ga19), is a cytoplasmic protein characterized by the presence of eight tetratricopeptide repeats (TPR). NARG1 plays a crucial role in cellular processes, particularly in acetylation regulation, as NARG1 interacts with ARD1 or ARD2 to form a complex that exhibits N-terminal (alpha) acetyltransferase activity. This activity is vital for cotranslational acetylation of ribosomal subunits, which is essential for proper protein synthesis and cellular function. NARG1 is highly expressed in rapidly dividing tissues such as bone marrow, testis, and embryonal brain, and NARG1 overexpression has been linked to papillary thyroid carcinomas. During apoptosis, both NARG1 and ARD1 are cleaved by caspases, leading to a decrease in acetyltransferase activity, which underscores NARG1′s importance in maintaining cell viability. NARG1 knockdown in HeLa cells has been shown to induce apoptosis, highlighting NARG1′s potential as a therapeutic target in cancer treatment.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NARG1 Antibody (D-7) References:
- N-methyl-D-aspartate receptors regulate a group of transiently expressed genes in the developing brain. | Sugiura, N., et al. 2001. J Biol Chem. 276: 14257-63. PMID: 11297529
- NATH, a novel gene overexpressed in papillary thyroid carcinomas. | Fluge, Ø., et al. 2002. Oncogene. 21: 5056-68. PMID: 12140756
- An evolutionarily conserved N-terminal acetyltransferase complex associated with neuronal development. | Sugiura, N., et al. 2003. J Biol Chem. 278: 40113-20. PMID: 12888564
- Identification and characterization of the human ARD1-NATH protein acetyltransferase complex. | Arnesen, T., et al. 2005. Biochem J. 386: 433-43. PMID: 15496142
- Interaction of N-terminal acetyltransferase with the cytoplasmic domain of beta-amyloid precursor protein and its effect on A beta secretion. | Asaumi, M., et al. 2005. J Biochem. 137: 147-55. PMID: 15749829
- Expression of N-acetyl transferase human and human Arrest defective 1 proteins in thyroid neoplasms. | Arnesen, T., et al. 2005. Thyroid. 15: 1131-6. PMID: 16279846
- Induction of apoptosis in human cells by RNAi-mediated knockdown of hARD1 and NATH, components of the protein N-alpha-acetyltransferase complex. | Arnesen, T., et al. 2006. Oncogene. 25: 4350-60. PMID: 16518407
- Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase. | Arnesen, T., et al. 2006. BMC Biochem. 7: 13. PMID: 16638120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NARG1 Antibody (D-7) | sc-365931 | 200 µg/ml | $316.00 | |||
NARG1 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-529516 | 200 µg Ab; 10 µg BP | $354.00 | |||
NARG1 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-522430 | 200 µg Ab, 40 µg BP | $354.00 | |||
NARG1 Antibody (D-7): m-IgG1 BP-HRP Bundle | sc-543555 | 200 µg Ab; 20 µg BP | $354.00 | |||
NARG1 Antibody (D-7) AC | sc-365931 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NARG1 Antibody (D-7) HRP | sc-365931 HRP | 200 µg/ml | $316.00 | |||
NARG1 Antibody (D-7) FITC | sc-365931 FITC | 200 µg/ml | $330.00 | |||
NARG1 Antibody (D-7) PE | sc-365931 PE | 200 µg/ml | $343.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 488 | sc-365931 AF488 | 200 µg/ml | $357.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 546 | sc-365931 AF546 | 200 µg/ml | $357.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 594 | sc-365931 AF594 | 200 µg/ml | $357.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 647 | sc-365931 AF647 | 200 µg/ml | $357.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 680 | sc-365931 AF680 | 200 µg/ml | $357.00 | |||
NARG1 Antibody (D-7) Alexa Fluor® 790 | sc-365931 AF790 | 200 µg/ml | $357.00 |