NANS Antibody (B-6): sc-374133

NANS Antibody (B-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects NANS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NANS (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NANS protein, also known as N-acetylneuraminic acid synthase, plays a crucial role in the biosynthesis of sialic acids, which are essential for numerous cellular recognition events and interactions. Sialic acids, a family of 9-carbon 2-keto-3-deoxy sugars, are typically found at the terminal ends of glycoproteins and glycolipids, influencing processes such as cell signaling, immune response, and pathogen recognition. NANS catalyzes the formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) through the utilization of specific substrates, highlighting NANS importance in maintaining cellular functions and communication. NANS consists of 359 amino acids and features an antifreeze protein-like domain, indicating a potential evolutionary conservation of function, as evidenced by 36% identity with the E. coli neuB protein. This conservation underscores NANS significance in both prokaryotic and eukaryotic systems, making anti-NANS antibody (B-6) a valuable tool for researchers studying sialic acid biosynthesis and its implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NANS Antibody (B-6) References:

1. Cloning and expression of the human N-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-D-glycero- D-galacto-nononic acid biosynthetic ability.  |  Lawrence, SM., et al. 2000. J Biol Chem. 275: 17869-77. PMID: 10749855
2. Molecular cloning and expression of the mouse N-acetylneuraminic acid 9-phosphate synthase which does not have deaminoneuraminic acid (KDN) 9-phosphate synthase activity.  |  Nakata, D., et al. 2000. Biochem Biophys Res Commun. 273: 642-8. PMID: 10873658
3. Cloning, expression, and characterization of sialic acid synthases.  |  Hao, J., et al. 2005. Biochem Biophys Res Commun. 338: 1507-14. PMID: 16274664
4. Solution structure of the antifreeze-like domain of human sialic acid synthase.  |  Hamada, T., et al. 2006. Protein Sci. 15: 1010-6. PMID: 16597820

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NANS Antibody (B-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NANS Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NANS Antibody (B-6) Specifications

Alternate Product Name: NANS

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: NANS

Epitope Species: human

Product Reactivity/Detection: NANS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NANS Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin