NALP2 Antibody (H-4): sc-166584
- NALP2 Antibody (H-4) is a mouse monoclonal IgG1 κ NALP2 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 671-690 within an internal region of NALP2 of human origin
- NALP2 Antibody (H-4) is recommended for detection of all isoforms of NALP2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NALP2 Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NALP2 Antibody (H-4) for WB applications. These reagents are now offered in bundles with NALP2 Antibody (H-4) (see ordering information below).
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NALP2 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NALP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NALP2 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NALP2, also known as PAN1 or PYPAF2, is a 1,062 amino acid protein that plays a critical role in regulating inflammatory responses by modulating nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathway activity. NALP2 inhibits NFKBIA degradation, thereby suppressing TNF- and CD40-induced NF-kB1 activity at the IKK complex level. This regulatory function maintains cellular homeostasis and prevents excessive inflammation. NALP2 associates with PYCARD to activate caspase-1, leading to proinflammatory cytokine IL-1β secretion. As part of the inflammasome complex with PYCARD, CARD8, and caspase-1, NALP2 activates proinflammatory caspases, highlighting its importance in immune responses. NALP2 shows predominant expression in lung, placenta, and thymus tissues, with lower expression in ovary, intestine, and brain, suggesting diverse physiological roles. NALP2 contains one DAPIN domain, nine leucine-rich repeats, and one NACHT domain, with DAPIN being essential for NF-kB1 activation suppression and IL-1β secretion in collaboration with caspase-1.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NALP2 Antibody (H-4) References:
- PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-kappaB and caspase-1 activation in macrophages. | Bruey, JM., et al. 2004. J Biol Chem. 279: 51897-907. PMID: 15456791
- PYPAF3, a PYRIN-containing APAF-1-like protein, is a feedback regulator of caspase-1-dependent interleukin-1beta secretion. | Kinoshita, T., et al. 2005. J Biol Chem. 280: 21720-5. PMID: 15817483
- Induction of toll-like receptors and NALP/PAN/PYPAF family members by modified oligonucleotides in lung epithelial carcinoma cells. | Drygin, D., et al. 2005. Oligonucleotides. 15: 105-18. PMID: 15989425
- Diffusion of alcohol upon application of neuroadenolysis of the pituitary gland (NALP). An experimental study using HRP and WGA-HRP in the cat. | Morimoto, M., et al. 1991. Fukuoka Igaku Zasshi. 82: 475-9. PMID: 1721037
- Mechanism of neuroadenolysis of the pituitary for cancer pain control. | Trouwborst, A., et al. 1984. Appl Neurophysiol. 47: 97-110. PMID: 6508283
- Relief of cancer pain in man: alcohol-induced neuroadenolysis vs. electrical stimulation of the pituitary gland. | Yanagida, H., et al. 1984. Pain. 19: 133-41. PMID: 6611531
- Neuroadenolysis of the pituitary. | Moricca, G., et al. 1981. Acta Anaesthesiol Belg. 32: 87-99. PMID: 7293713
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NALP2 Antibody (H-4) | sc-166584 | 200 µg/ml | $316.00 | |||
NALP2 Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-527172 | 200 µg Ab; 10 µg BP | $354.00 | |||
NALP2 Antibody (H-4): m-IgG1 BP-HRP Bundle | sc-532545 | 200 µg Ab; 20 µg BP | $354.00 | |||
NALP2 Antibody (H-4) AC | sc-166584 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NALP2 Antibody (H-4) HRP | sc-166584 HRP | 200 µg/ml | $316.00 | |||
NALP2 Antibody (H-4) FITC | sc-166584 FITC | 200 µg/ml | $330.00 | |||
NALP2 Antibody (H-4) PE | sc-166584 PE | 200 µg/ml | $343.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 488 | sc-166584 AF488 | 200 µg/ml | $357.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 546 | sc-166584 AF546 | 200 µg/ml | $357.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 594 | sc-166584 AF594 | 200 µg/ml | $357.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 647 | sc-166584 AF647 | 200 µg/ml | $357.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 680 | sc-166584 AF680 | 200 µg/ml | $357.00 | |||
NALP2 Antibody (H-4) Alexa Fluor® 790 | sc-166584 AF790 | 200 µg/ml | $357.00 | |||
NALP2 (H-4) Neutralizing Peptide | sc-166584 P | 100 µg/0.5 ml | $68.00 |