NADSYN1 Antibody (3E3): sc-100485

NADSYN1 Antibody (3E3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NADSYN1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-NADSYN1 antibody (3E3) is available as the non-conjugated format. NADSYN1, also known as NAD synthetase 1, plays a crucial role in the synthesis of nicotinamide adenine dinucleotide (NAD), a vital cofactor involved in numerous cellular processes, including metabolic redox reactions and cell signaling. This 706 amino acid protein features a carbon-nitrogen hydrolase domain and is a member of the NAD synthetase family, which is essential for the ATP-dependent conversion of deamido-NAD+ to free NAD+. NADSYN1 is predominantly expressed in the testis, kidney, liver, and small intestine, highlighting its importance in various metabolic pathways. Notably, NADSYN1 exists as a homohexamer and utilizes both ammonia and glutamate as amide donors, which is significant for enzymatic activity. Furthermore, NADSYN1 expression in human promyelocytic leukemia and glioma cell lines suggests a potential involvement in tumorigenesis, making NADSYN1 a protein of interest for cancer research.

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NADSYN1 Antibody (3E3) References:

1. Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency.  |  Hara, N., et al. 2003. J Biol Chem. 278: 10914-21. PMID: 12547821
2. Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements.  |  Jauch, R., et al. 2005. J Biol Chem. 280: 15131-40. PMID: 15699042
3. Glutamine amidotransferase activity of NAD+ synthetase from Mycobacterium tuberculosis depends on an amino-terminal nitrilase domain.  |  Bellinzoni, M., et al. 2005. Res Microbiol. 156: 173-7. PMID: 15748981
4. Glutamine-dependent NAD+ synthetase. How a two-domain, three-substrate enzyme avoids waste.  |  Wojcik, M., et al. 2006. J Biol Chem. 281: 33395-402. PMID: 16954203

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NADSYN1 Antibody (3E3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NADSYN1 Antibody (3E3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NADSYN1 Antibody (3E3) Specifications

Alternate Product Name: NADSYN1

Product Clone Name: 3E3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 609-707

Epitope Target: NADSYN1

Epitope Species: human

Product Reactivity/Detection: NADSYN1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NADSYN1 Antibody (3E3): N/A