NaBC1 Antibody (F-4): sc-393740
- NaBC1 Antibody (F-4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of NaBC1 of human origin
- Anti-NaBC1 Antibody (F-4) is recommended for detection of NaBC1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NaBC1 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NaBC1 Antibody (F-4) for WB applications. These reagents are now offered in bundles with NaBC1 Antibody (F-4) (see ordering information below).
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NaBC1 Antibody (F-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NaBC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NaBC1 Antibody (F-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NaBC1, also known as Novel amplified in breast cancer 1, plays a crucial role in cellular processes and is notably amplified in many breast carcinoma cases. NaBC1 is primarily located in the cytoplasm as a detergent-stable homodimer, suggesting potential involvement in intracellular signaling pathways. NaBC1 has been shown to interact with proteins such as DYNLL1 and DYNLL2, which are involved in various cellular functions, including motor protein activity and cellular transport. Breast tumor lines exhibiting amplification of the 20q13.2 region show markedly elevated levels of NaBC1 compared to other breast cancer lines that do not overexpress NaBC1 mRNA, yet this upregulation does not correlate with increased growth rates or anchoring abilities, suggesting that NaBC1′s oncogenic properties may operate through distinct mechanisms compared to other oncogenes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NaBC1 Antibody (F-4) References:
- NABC1 (BCAS1): alternative splicing and downregulation in colorectal tumors. | Correa, RG., et al. 2000. Genomics. 65: 299-302. PMID: 10857754
- Cloning and characterization of a novel synaptosome-enriched mRNA that encodes 31 kDa protein. | Ishimoto, T., et al. 2002. Biochim Biophys Acta. 1579: 189-95. PMID: 12427555
- Elevated expression levels of NCOA3, TOP1, and TFAP2C in breast tumors as predictors of poor prognosis. | Zhao, C., et al. 2003. Cancer. 98: 18-23. PMID: 12833450
- Characterization of the novel amplified in breast cancer-1 (NABC1) gene product. | Beardsley, DI., et al. 2003. Exp Cell Res. 290: 402-13. PMID: 14567997
- Prognostic relevance of 20q13 gains in sporadic colorectal cancers: a FISH analysis. | Aust, DE., et al. 2004. Scand J Gastroenterol. 39: 766-72. PMID: 15513363
- Genomic array and expression analysis of frequent high-level amplifications in adenocarcinomas of the gastro-esophageal junction. | van Dekken, H., et al. 2006. Cancer Genet Cytogenet. 166: 157-62. PMID: 16631473
- Genome-wide array-based comparative genomic hybridization analysis of pancreatic adenocarcinoma: identification of genetic indicators that predict patient outcome. | Loukopoulos, P., et al. 2007. Cancer Sci. 98: 392-400. PMID: 17233815
- A comparative study of genome-wide SNP, CGH microarray and protein expression analysis to explore genotypic and phenotypic mechanisms of acquired antiestrogen resistance in breast cancer. | Johnson, N., et al. 2008. Breast Cancer Res Treat. 111: 55-63. PMID: 17899364
- Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. | Collins, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 8703-8. PMID: 9671742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NaBC1 Antibody (F-4) | sc-393740 | 200 µg/ml | $316.00 | |||
NaBC1 Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-527708 | 200 µg Ab; 10 µg BP | $354.00 | |||
NaBC1 Antibody (F-4): m-IgG1 BP-HRP Bundle | sc-533081 | 200 µg Ab; 20 µg BP | $354.00 | |||
NaBC1 Antibody (F-4) AC | sc-393740 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NaBC1 Antibody (F-4) HRP | sc-393740 HRP | 200 µg/ml | $316.00 | |||
NaBC1 Antibody (F-4) FITC | sc-393740 FITC | 200 µg/ml | $330.00 | |||
NaBC1 Antibody (F-4) PE | sc-393740 PE | 200 µg/ml | $343.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 488 | sc-393740 AF488 | 200 µg/ml | $357.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 546 | sc-393740 AF546 | 200 µg/ml | $357.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 594 | sc-393740 AF594 | 200 µg/ml | $357.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 647 | sc-393740 AF647 | 200 µg/ml | $357.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 680 | sc-393740 AF680 | 200 µg/ml | $357.00 | |||
NaBC1 Antibody (F-4) Alexa Fluor® 790 | sc-393740 AF790 | 200 µg/ml | $357.00 |