NAB1 Antibody (A-8): sc-137084
- NAB1 Antibody (A-8) is a mouse monoclonal IgG2b κ NAB1 antibody provided at 200 µg/ml
- raised against amino acids 281-486 mapping at the C-terminus of NAB1 of human origin
- NAB1 Antibody (A-8) is recommended for detection of NAB1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NAB1 Antibody (A-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NAB1 Antibody (A-8) for WB applications. These reagents are now offered in bundles with NAB1 Antibody (A-8) (see ordering information below).
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NAB1 Antibody (A-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects NAB1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NAB1 (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NAB1 protein plays a crucial role in transcriptional regulation, functioning as a corepressor that interacts with DNA-bound transcription factors like Egr1/NGFI-A. This interaction is vital for the precise control of gene expression, as NAB1 and its homolog NAB2 are essential for modulating the activity of Egr1, which is involved in critical cellular processes such as cell division, differentiation, and apoptosis. The structure of NAB1 includes an amino-terminal NAB-conserved domain 1 (NCB1) necessary for binding to NGFI-A and a carboxy-terminal NCD2 that mediates its repressor function. NAB1 is constitutively expressed and localized exclusively in the nucleus, underscoring its importance in regulating gene expression and maintaining cellular homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NAB1 Antibody (A-8) References:
- A novel activation function for NAB proteins in EGR-dependent transcription of the luteinizing hormone beta gene. | Sevetson, BR., et al. 2000. J Biol Chem. 275: 9749-57. PMID: 10734128
- The transcriptional repressor Nab1 is a specific regulator of pathological cardiac hypertrophy. | Buitrago, M., et al. 2005. Nat Med. 11: 837-44. PMID: 16025126
- Therapeutic applications of the transcriptional corepressor proteins NAB1 and NAB2 in regenerative medicine. | Braddock, M., et al. 2000. IDrugs. 3: 783-7. PMID: 16080053
- NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. | Mussgnug, JH., et al. 2005. Plant Cell. 17: 3409-21. PMID: 16284312
- Comparative proteomics of high light stress in the model alga Chlamydomonas reinhardtii. | Förster, B., et al. 2006. Proteomics. 6: 4309-20. PMID: 16800035
- Identification of NAB1, a repressor of NGFI-A- and Krox20-mediated transcription. | Russo, MW., et al. 1995. Proc Natl Acad Sci U S A. 92: 6873-7. PMID: 7624335
- Transcriptional activity of the zinc finger protein NGFI-A is influenced by its interaction with a cellular factor. | Russo, MW., et al. 1993. Mol Cell Biol. 13: 6858-65. PMID: 8413279
- NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli. | Svaren, J., et al. 1996. Mol Cell Biol. 16: 3545-53. PMID: 8668170
- Nab1, a corepressor of NGFI-A (Egr-1), contains an active transcriptional repression domain. | Swirnoff, AH., et al. 1998. Mol Cell Biol. 18: 512-24. PMID: 9418898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NAB1 Antibody (A-8) | sc-137084 | 200 µg/ml | $316.00 | |||
NAB1 Antibody (A-8): m-IgGκ BP-HRP Bundle | sc-521421 | 200 µg Ab, 40 µg BP | $354.00 | |||
NAB1 Antibody (A-8): m-IgG2b BP-HRP Bundle | sc-548894 | 200 µg Ab; 10 µg BP | $354.00 | |||
NAB1 Antibody (A-8) AC | sc-137084 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NAB1 Antibody (A-8) HRP | sc-137084 HRP | 200 µg/ml | $316.00 | |||
NAB1 Antibody (A-8) FITC | sc-137084 FITC | 200 µg/ml | $330.00 | |||
NAB1 Antibody (A-8) PE | sc-137084 PE | 200 µg/ml | $343.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 488 | sc-137084 AF488 | 200 µg/ml | $357.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 546 | sc-137084 AF546 | 200 µg/ml | $357.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 594 | sc-137084 AF594 | 200 µg/ml | $357.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 647 | sc-137084 AF647 | 200 µg/ml | $357.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 680 | sc-137084 AF680 | 200 µg/ml | $357.00 | |||
NAB1 Antibody (A-8) Alexa Fluor® 790 | sc-137084 AF790 | 200 µg/ml | $357.00 |