ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9): sc-58628

Na+/K+-ATPase α Antibody (M7-PB-E9) is a mouse monoclonal IgG1 kappa light chain antibody, specifically engineered to recognize the Na+/K+-ATPase α subunit. Na+/K+-ATPase α monoclonal antibody (M7-PB-E9) is raised against the ovine Na+/K+-ATPase α protein and effectively detects α isoforms from mouse and human origins, as well as the α3 isoform from rat origin, through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Additionally, Na+/K+-ATPase α (M7-PB-E9) antibody shows reactivity with other species including ovine, bovine, porcine, and canine, while not cross-reacting with the α1 isoform in Xenopus or chicken, or with the α2 or α3 isoforms in rat. Na+/K+-ATPase is a crucial membrane-bound enzyme composed of α, β, and γ subunits, responsible for maintaining essential electrochemical gradients by actively transporting three sodium ions out of the cell and two potassium ions into the cell. This activity is vital for various cellular processes such as regulating cellular volume, maintaining resting membrane potential, and facilitating secondary active transport of other solutes. The α subunit serves as the catalytic core, housing binding sites for ATP and the ions, while the β subunit ensures proper folding and membrane localization, and the γ subunit modulates the enzyme′s affinity for ATP. Na+/K+-ATPase interacts with several regulatory proteins, including ankyrin, which links to the cytoskeleton, and Src kinase, which is involved in signal transduction pathways. These interactions are essential for enzyme regulation and cellular signaling mechanisms. Na+/K+-ATPase α (M7-PB-E9) monoclonal antibody is an indispensable tool for researchers investigating ion transport dynamics, cellular homeostasis, and the intricate protein interactions that govern physiological processes, providing reliable detection across multiple species and experimental techniques.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9) Specifications

Alternate Product Name: ATP1A1

Product Clone Name: M7-PB-E9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Na⁺/K⁺-ATPase α

Epitope Species: ovine

Product Reactivity/Detection: all α isoforms of Na+/K+-ATPase of mouse and human origin and Na+/K+-ATPase α3 of rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: ovine, bovine, porcine and canine

Additional Non-Mammalian Reactivity: chicken

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred alpha 1 Sodium Potassium ATPase/ATP1A1 antibody is alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody (C464.6): sc-21712.