N-WASP Antibody (C-1): sc-271484

N-WASP Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects N-WASP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). N-WASP (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. N-WASP monoclonal antibody (C-1) is specific for an epitope mapping between amino acids 18-49 near the N-terminus of N-WASP of human origin. N-WASP plays a crucial role in actin polymerization and cytoskeletal organization, which are essential processes for various cellular functions, including cell motility, shape maintenance, and intracellular transport. N-WASP is vital for the Cdc42-induced formation of filopodia, which are slender, actin-rich projections that extend from the cell surface and are involved in sensing the extracellular environment and facilitating cell movement. N-WASP interaction with the Arp2/3 complex is critical for enhancing actin polymerization, thereby promoting dynamic reorganization of the cytoskeleton. This dynamic reorganization is particularly important in neural tissues, where N-WASP is predominantly expressed, as N-WASP contributes to processes such as neurite outgrowth and synaptic plasticity. Understanding N-WASP function and regulatory mechanisms is essential for elucidating the underlying causes of conditions such as Wiskott-Aldrich syndrome, which is characterized by immunodeficiency and other systemic issues due to mutations affecting actin dynamics.

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N-WASP Antibody (C-1) References:

1. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.  |  Rohatgi, R., et al. 1999. Cell. 97: 221-31. PMID: 10219243
2. Distinct phosphorylation requirements regulate cortactin activation by TirEPEC and its binding to N-WASP.  |  Nieto-Pelegrin, E. and Martinez-Quiles, N. 2009. Cell Commun Signal. 7: 11. PMID: 19419567
3. N-WASP is a novel regulator of hair-follicle cycling that controls antiproliferative TGF{beta} pathways.  |  Lefever, T., et al. 2010. J Cell Sci. 123: 128-40. PMID: 20016073
4. The expression of CFL1 and N-WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features.  |  Wang, WS., et al. 2010. Dis Esophagus. 23: 512-21. PMID: 20095995
5. N-WASp is required for Schwann cell cytoskeletal dynamics, normal myelin gene expression and peripheral nerve myelination.  |  Jin, F., et al. 2011. Development. 138: 1329-37. PMID: 21385763
6. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.  |  Symons, M., et al. 1996. Cell. 84: 723-34. PMID: 8625410
7. Defects in Wiskott-Aldrich syndrome blood cells.  |  Remold-O'Donnell, E., et al. 1996. Blood. 87: 2621-31. PMID: 8639877
8. Studies of the expression of the Wiskott-Aldrich syndrome protein.  |  Stewart, DM., et al. 1996. J Clin Invest. 97: 2627-34. PMID: 8647957
9. N-WASP, a novel actin-depolymerizing protein, regulates the cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.  |  Miki, H., et al. 1996. EMBO J. 15: 5326-35. PMID: 8895577
10. Direct binding of the verprolin-homology domain in N-WASP to actin is essential for cytoskeletal reorganization.  |  Miki, H. and Takenawa, T. 1998. Biochem Biophys Res Commun. 243: 73-8. PMID: 9473482

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-N-WASP Antibody (C-1) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-N-WASP Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

N-WASP Antibody (C-1) Specifications

Alternate Product Name: Neural Wiskott-Aldrich Syndrome Protein

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 18-49

Epitope Target: N-WASP

Epitope Species: human

Product Reactivity/Detection: N-WASP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for N-WASP Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin