N-type Ca⁺⁺ CP α1B CRISPR/Cas9 KO Plasmid (m): sc-419401

Overview

Cacna1b encodes the α1B pore-forming subunit of N-type Ca²⁺ channels (CaV2.2), a high-voltage–activated calcium channel enriched at presynaptic terminals where it couples membrane depolarization to Ca²⁺ influx and neurotransmitter release. CaV2.2 activity integrates with synaptic vesicle exocytosis machinery, calcium-dependent kinase/phosphatase signaling, and short- and long-term synaptic plasticity pathways. In mouse nervous system models, altered CACNA1B function has been linked to dysregulated neuronal excitability and sensory neurotransmission, supporting relevance to studies of pain circuitry, seizure susceptibility, and neuropsychiatric phenotypes. Because CaV2.2 shapes presynaptic calcium microdomains, perturbation of Cacna1b provides a direct handle on activity-dependent signaling and network-level communication.

N-type Ca++ CP α1B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cacna1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cacna1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cacna1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish N-type Ca++ CP α1B protein expression.

This CRISPR knockout system enables efficient generation of Cacna1b-deficient cell models for investigation of N-type Ca++ CP α1B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Cacna1b exon(s) critical for N-type Ca++ CP α1B function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Cacna1b genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by N-type Ca++ CP α1B CRISPR/Cas9 KO Plasmid (m) and N-type Ca++ CP α1B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cacna1b locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by N-type Ca++ CP α1B HDR Plasmid (m) and N-type Ca++ CP α1B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cacna1b homology arms to support homology-directed repair at defined Cacna1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.