Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
N-Ras CRISPR/Cas9 KO Plasmid (m): sc-421960
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- N-Ras CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the N-Ras genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: N-Ras Antibody (F155): sc-31
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-Ras CRISPR/Cas9 KO Plasmid (m) | sc-421960 | 20 µg | $397.00 |
Overview
Mouse Nras encodes N-Ras, a membrane-associated small GTPase that cycles between GDP- and GTP-bound states to transduce signals from receptor tyrosine kinases and other upstream cues. Activated N-Ras engages core Ras effector pathways including RAF–MEK–ERK (MAPK) and PI3K–AKT, coordinating proliferation, differentiation, cytoskeletal dynamics, and survival. Tight control of N-Ras activity is essential for normal hematopoietic and developmental signaling, and perturbation of Ras pathway flux is broadly relevant to oncogenic transformation and inflammatory microenvironment responses. Nras therefore serves as a key node for dissecting Ras-driven network rewiring and pathway crosstalk in mouse cellular and disease models.
N-Ras CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nras gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nras together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nras open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish N-Ras protein expression.
This CRISPR knockout system enables efficient generation of Nras-deficient cell models for investigation of N-Ras signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nras exon(s) critical for N-Ras function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nras genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by N-Ras CRISPR/Cas9 KO Plasmid (m) and N-Ras CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nras locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by N-Ras HDR Plasmid (m) and N-Ras HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nras homology arms to support homology-directed repair at defined Nras target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.