N-Ras Antibody (F155): sc-31
- Anti-N-Ras Antibody (F155) is a mouse monoclonal IgG1 κ N-Ras antibody, cited in 240 publications, provided at 100 µg/ml
- raised against recombinant N-Ras p21 of human origin
- recommended for detection of N-Ras p21 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-N-Ras Antibody (F155) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for N-Ras Antibody (F155) for WB and IHC(P) applications. These reagents are now offered in bundles with N-Ras Antibody (F155) (see ordering information below).
N-Ras Antibody (F155) is a mouse monoclonal IgG1 kappa light chain antibody, specifically raised against recombinant human N-Ras p21, and demonstrates reactivity with N-Ras proteins from mouse, rat, and human origins in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). The N-Ras protein is a small GTPase belonging to the Ras family, playing a crucial role in cellular signal transduction pathways that regulate cell proliferation, differentiation, and survival. N-Ras functions as a molecular switch by cycling between an active GTP-bound state and an inactive GDP-bound state, a process regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Post-translational modifications of N-Ras, such as farnesylation and palmitoylation at its C-terminal CAAX motif, are essential for proper localization to the plasma membrane and interaction with downstream effectors. These lipid modifications enable N-Ras to anchor to cellular membranes, which is critical for transmitting signals that influence cell growth and differentiation. Disruptions in these post-translational modifications can lead to mislocalization and aberrant signaling of N-Ras, contributing to oncogenesis and the progression of various cancers. N-Ras (F155) antibody is a valuable tool for researchers studying the expression, localization, and function of N-Ras in both normal physiology and disease states, aiding in the advancement of understanding cellular signaling mechanisms and the development of targeted therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
N-Ras Antibody (F155) References:
- The dark side of Ras: regulation of apoptosis. | Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
- Ras signaling in prostate cancer progression. | Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
- Human RAS superfamily proteins and related GTPases. | Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
- Oncogenic Ras in tumour progression and metastasis. | Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
- Ras plasma membrane signalling platforms. | Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
- Ras signaling on the Golgi. | Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
- Compartmentalized Ras/MAPK signaling. | Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
- Ras, PI(3)K and mTOR signalling controls tumour cell growth. | Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
- Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses. | Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
N-Ras Antibody (F155) | sc-31 | 100 µg/ml | $316.00 | |||
N-Ras Antibody (F155): m-IgG Fc BP-HRP Bundle | sc-551590 | 100 µg Ab; 10 µg BP | $354.00 | |||
N-Ras Antibody (F155): m-IgG1 BP-HRP Bundle | sc-551589 | 100 µg Ab; 20 µg BP | $354.00 | |||
N-Ras Antibody (F155): m-IgGκ BP-HRP Bundle | sc-551591 | 100 µg Ab; 40 µg BP | $354.00 | |||
N-Ras Antibody (F155) AC | sc-31 AC | 500 µg/ml, 25% agarose | $416.00 | |||
N-Ras Antibody (F155) HRP | sc-31 HRP | 200 µg/ml | $316.00 | |||
N-Ras Antibody (F155) FITC | sc-31 FITC | 200 µg/ml | $330.00 | |||
N-Ras Antibody (F155) PE | sc-31 PE | 200 µg/ml | $343.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 488 | sc-31 AF488 | 200 µg/ml | $357.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 546 | sc-31 AF546 | 200 µg/ml | $357.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 594 | sc-31 AF594 | 200 µg/ml | $357.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 647 | sc-31 AF647 | 200 µg/ml | $357.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 680 | sc-31 AF680 | 200 µg/ml | $357.00 | |||
N-Ras Antibody (F155) Alexa Fluor® 790 | sc-31 AF790 | 200 µg/ml | $357.00 |